Effect of rest-grazing on soil microbial community functional diversity in Stipa baicalensis steppe

doi:10.11686/cyxb20130603

Abstract

Abstract: The changes in soil microbial community functional diversity and microbial biomass under different rest-grazing years (RG_3a, RG_6a and RG_9a) in Stipa baicalensis steppe were investigated and compared with zero rest grazing grassland (RG₀) using the Biolog-ECO micro-plate technique and chloroform fumigation extraction method. The soil microbial metabolic activity significantly increased after rest grazing. The average well color development (AWCD), which represented soil microbial metabolic activity, followed the order of RG_6a>RG_9a>RG_3a>RG₀. The AWCD of RG_6a and RG_9a showed no significant difference, but both were significantly higher than that of RG₀ (P<0.05). RG_3a had no significant difference from RG₀ (P>0.05). The RG_9a had the highest Shannon index (H), substrate evenness (E) and Simpson’s dominance (D) of soil microbial community. The Shannon index (H) of RG_9a was significantly (P<0.05) different from those under other treatments. Substrate evenness (E) was not significantly different from each other; Simpson’s Dominance (D) of RG_9a was not significantly different from those of RG_3a and RG_6a, but significantly different from RG₀. Principal component analysis (PCA) showed that the RG₀, RG_3a and RG_6a had a similar carbon source utilization mode and the metabolic function differed from that of RG_9a. The carbon sources most used by soil microorganisms were carbohydrates, amino acids, and lipids. Soil microbial biomass increased gradually with more rest grazing years. RG_9a had the highest soil microbial biomass C (590.20 mg/kg) and soil microbial biomass N (72.86 mg/kg). Correlation analysis indicated that the AWCD had a significantly (P<0.05) positive correlation with the Shannon index (H) and a highly significantly (P<0.01) positive correlation with Simpson’s dominance (D). The Shannon index had a highly significantly (P<0.01) positive correlation with the Simpson’s dominance (D). The Shannon index (H) and Simpson’s dominance (D) both showed a significantly (P<0.05) positive correlation with soil microbial biomass C and there was also a significantly (P<0.05) positive correlation between the Shannon index (H) and soil microbial biomass N (P<0.05). Therefore, rest grazing enhanced the soil microbial metabolic function which was beneficial to the reproduction of soil microorganisms, thereby promoting an increase of soil microbial biomass carbon and nitrogen content.

CLC Number:

LI Yu-jie, LI Gang, SONG Xiao-long, ZHAO Jian-ning, XIU Wei-ming, YANG Dian-lin. Effect of rest-grazing on soil microbial community functional diversity in Stipa baicalensis steppe[J]. Acta Prataculturae Sinica, 2013, 22(6): 21-30.

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