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Acta Prataculturae Sinica ›› 2013, Vol. 22 ›› Issue (6): 150-158.DOI: 10.11686/cyxb20130619

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Screening, identification and solubilizing properties of the endophytic phosphate-solubilizingbacteria isolated from Anaphalis lactea

LI Zhen-dong1,2, CHEN Xiu-rong1, YANG Cheng-de1, LI Peng1   

  1. 1.Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Ministry of Education; College of Pratacultural Science, Gansu Agricultural University; Pratacultural Engineering Laboratory of Gansu Province; Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
    2.Institute of Rural Development, Gansu Academy of Social Sciences, Lanzhou 730070, China
  • Online:2013-12-20 Published:2013-12-20

Abstract: To screen and identify endophytic phosphate-solubilizing bacteria, endophytic bacteria were isolated from Anaphalis lacteal, the dominant grass of the alpine grasslands in the Eastern Qilian Mountains. They were screened using Pikovaskaia’s culture medium (PKO), and identified by physiological and biochemical tests and 16S rDNA analysis. How the endophytic phosphate-solubilizing bacteria affect the growth of corn was also studied. Four endophytic phosphate-solubilizing bacterial strains were isolated on media with phosphate (P2O5) contents between 65.24 and 315.36 mg/L. Isolate RA8 had the highest phosphate-solubilizing capacity and was identified as Paracoccus sp. on the basis of morphological, physiological, and biochemical characteristics, and on phylogenetic analysis of the 16S rDNA gene sequence. RA8 can produce acid, but the phosphate (P2O5) content had no correlation with the pH value of the culture media. It was the non-acid metabolic substances of RA8 that played a role in the mechanism of dissolving phosphate. RA8 can cooperate with indigenous microorganisms in the soil, and as a result, RA8 has a strong phosphate-solubilizing capacity in the soil. The plant biomass of the corn treated with RA8 was significantly (P<0.05) higher than that of the control at the 70th day and 100th day, and the plant height of the corn treated with RA8 was significantly (P<0.01) higher than that of the control at the 100th day showing that RA8 can significantly promote the growth of corn.

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