Effect of fermention on ethanol production from fresh sweet corn stalks

doi:10.11686/cyxb20140219

Abstract

Abstract: Corn stalks are an important resource that is abundant and cheap for fuel ethanol production. This study took curtal fresh sweet corn stalk as raw material with, yeasts (J), lactic acid bacteria (R) and cellulase (E) as additives. The additive treatments were: control (no additive), J, JR (J+R), JE (J+E), JER (J+E+R), MJ (J addition after sterilizing) and MJER (JER addition after sterilizing). The treated materials were sealed, and fermented for 10, 20, 30 and 40 days to study the optimum fermentation time and additives for ethanol production. The contents of water soluble carbohydrates (WSC) in sweet corn stalk significantly decreased with prolonging of fermentation times and were lower in the J, JR and MJ treatments than in the control (P<0.05). Fermentation time and additives had no significant effect on cellulose and hemicellulose contents. The ethanol yield tended to decrease with fermentation time from 10 to 40 days and was the highest (17.89% DM) under the following conditions: sterilized stalk and adding yeasts, fermenting for 10 d.

CLC Number:

GAO Rui-fang, ZHANG Jian-guo. Effect of fermention on ethanol production from fresh sweet corn stalks[J]. Acta Prataculturae Sinica, 2014, 23(2): 154-159.

References

[1] 于建仁, 张曾, 迟聪聪. 生物质精炼与制浆造纸工业相结合的研究[J]. 中国造纸学报, 2008, 23(1): 80-84.
[2] Lee J. Biological conversion of lignocellulosic biomass to ethanol[J]. Journal of Biotechnology, 1997, 56(1): 1-24.
[3] Nagle N, Ibsen K, Jennings E. A process economic approach to develop a dilute-acid cellulose hydrolysis process to produce ethanol from biomass[J]. Applied Biochemistry and Biotechnology, 1999, 79(1-3): 595-607.
[4] 黎先发, 张颖, 罗学刚. 利用木质纤维素生产燃料酒精研究进展[J]. 现代化工, 2009, 29(1): 20-26.
[5] 毕于运. 秸秆资源评价与利用研究[D]. 北京: 中国农业科学院, 2010.
[6] 彭云玲, 李伟丽, 王坤泽, 等. NaCl胁迫对玉米耐盐系与盐敏感系萌发和幼苗生长的影响[J]. 草业学报, 2012, 21(4): 62-71.
[7] 武冬梅, 李冀新, 孙新纪. 纤维素类物质发酵生产燃料乙醇的研究进展[J]. 酿酒科技, 2007, (4): 116-120.
[8] 柴民杰, 李磊, 李金民. 我国秸秆的利用现状及发展趋势分析[J]. 硅谷, 2009, (19): 158.
[9] 邢廷铣. 秸秆饲料的有效利用[J]. 饲料研究, 1995, (6): 2-3.
[10] 杏艳. 秸秆类生物质的固态酶解预处理及发酵产氢的研究[D]. 郑州: 郑州大学, 2008.
[11] 刘小杰, 郝向丽. 利用稻草粉同时糖化发酵的初步研究[J]. 粮油加工, 2007, (3): 86-88.
[12] 张强, 陆军, 侯霖, 等. 玉米秸秆发酵法生产燃料酒精的研究进展[J]. 饲料工业, 2005, 26(9): 20-23.
[13] 王子明. 广东甜玉米占半壁江山举足轻重[EB/OL]. http://www.nfncb.cn/article-68325-1.html, 2013-4-9.
[14] 张白鸽, 陈琼贤, 罗少波, 等. 广州地区甜玉米测土配方施肥指标体系研究[J]. 广东农业科学, 2012, (20): 46-48.
[15] 曾晓花, 万忠, 尹艳, 等. 2011年广东甜玉米产业发展现状分析[J]. 广东农业科学, 2012, (3): 12-14.
[16] 张媛, 王子明, 刘鹏飞, 等. 广东省甜玉米育种及栽培研究成果与发展思路[J]. 仲恺农业工程学院学报, 2012, 25(2): 67-71.
[17] 康利平, 刘莉, 刘萍, 等. 甜高粱茎秆固态发酵生产燃料乙醇研究[J]. 农业工程学报, 2008, 24(7): 181-184.
[18] 王菁莎. 纤维素酶的制备及玉米秸秆固态发酵酒精的研究[D]. 保定: 河北农业大学, 2006.
[19] 吕文龙, 刁其玉, 闫贵龙. 布氏乳杆菌对青玉米秸青贮发酵品质和有氧稳定性的影响[J]. 草业学报, 2011, 20(3): 143-148.
[20] 张丽英. 饲料分析及饲料质量检测技术[M]. 北京: 中国农业科技出版社, 2003: 46-75.
[21] 韩雅珊. 食品化学实验指导[M]. 北京: 北京农业大学出版社, 1996: 26-29.
[22] 靳春林, 张宏志. 毛细管柱气相色谱法快速测定中药酒中的乙醇量[J]. 山东化工, 1998, (5): 41-43.
[23] 韩立英, 玉柱. 3种乳酸菌制剂对苜蓿和羊草的青贮效果[J]. 草业科学, 2009, 26(2): 66-77.
[24] Zhang J G, Kumai S. Effluent and aerobic stability of cellulose and LAB-treated silage of napier grass (Pennisetum purpureum Schum)[J]. Asian-Australasian Journal of Animal Science, 2000, 8: 1035-1188.
[25] 玉柱, 贾玉山. 牧草饲草加工与贮藏[M]. 北京: 中国农业大学出版社, 2010: 77.
[26] 任天宝. 玉米秸秆发酵生产燃料乙醇的试验研究[D]. 郑州: 河南农业大学, 2007.
[27] 谢慧. 以玉米秸秆为原料同步糖化发酵生产燃料乙醇[J]. 生物学杂志, 2011, 28(5): 91-97.
[28] 张强, 戈兴炜, Anders T, 等. 利用树干毕赤酵母发酵玉米秸秆制备燃料酒精[J]. 化工进展, 2010, 29(12): 2270-2273.
[29] 张晓庆, 樊丽娟. 不同发酵型乳酸菌在饲料青贮中应用的研究进展[J]. 饲料与营养, 2008, (5): 24-25.
[30] 王奇, 余成群, 李志华, 等. 添加酶和乳酸菌制剂对西藏苇状羊茅和箭筈豌豆混合青贮发酵品质的影响[J]. 草业学报, 2012, 21(4): 186-191.
[31] 申成利, 陈明霞, 李国栋, 等. 添加乳酸菌和菠萝皮对柱花草青贮品质的影响[J]. 草业学报, 2012, 21(4): 192-197.
[32] 王洪志. 奶啤生产中乳酸菌对酵母菌发酵作用的研究[J]. 中国酿造, 2009, (6): 134-136.
[33] 孙万儒. 酵母菌[J]. 生物学通报, 2007, 42(11): 5-10.
[34] 赖智乐, 常春, 马晓建. 同步糖化发酵在纤维乙醇生产中的研究进展[J]. 酿酒科技, 2009, (7): 86-90.
[35] Lin Y, Tanaka S. Ethanol fermentation from biomass resources: current state and prospects[J]. Applied Microbiology and Biotechnology, 2006, 69(6): 627-642.
[36] 阴春梅, 刘忠, 齐宏升. 生物质发酵生产乙醇的研究进展[J]. 酿酒科技, 2007, (1): 87-90.
[37] Claassen P A M, Van Lier J B, Contreras A M L, et al. Utilization of biomass for the supply of energy carriers[J]. Applied Microbiology Biotechnology, 1999, 52(6): 741-755.
[38] Supple S G, Joachimsthal E L, Dunn N W, et al. Isolation and preliminary characterization of a Zymomonas mobilis mutant with an altered preference for xylose and glucose utilization[J]. Biotechnology Letters, 2000, 22(2): 157-164.
[39] Jeffries T W, Kurtzman C P. Strain selection, taxonomy, and genetics of xylose-fermenting yeasts[J]. Enzyme Microbial Technology, 1994, 16(11): 922-932.
[40] Nigam J N. Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicelluloses acid prehydrolysate[J]. Journal of Applied Microbiology, 2001, 90(2): 208-215.
[41] 曹秀华, 阮奇城, 林海红, 等. 纤维燃料乙醇生产中木糖发酵的研究进展[J]. 中国麻业科学, 2010, 32(3): 166-182.
[42] 田沈, 姚莹秋, 蔺增曦, 等. 木质纤维素稀酸水解糖液乙醇发酵研究进展[J]. 微生物学通报, 2007, 34(2): 355-358.
[43] 安宏. 纤维素稀酸水解制取燃料酒精的试验研究[D]. 杭州: 浙江大学, 2005.
[44] Ballesteros M, Oliva J M, Negro M J, et al. Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SFS) with Kluyveromyces marxianus CECT 10875[J]. Process Biochemistry, 2004, 39(12): 1843-1848.
[45] Jeffries T W, Jin Y S. Ethanol and thermotolerance in the bioconversion of xylose by yeasts[J]. Advances in Applied Microbiology, 2000, 47: 221-268.
[46] 刘海臣. 稻草秸秆同步糖化发酵制备纤维素乙醇的研究[D]. 徐州: 中国矿业大学, 2010.
[47] EI-Zawawy W K, Ibrahim M M, Abdel-Fattah Y R, et al. Acid and enzyme hydrolysis to convert pretreated lignocellulosic materials into glucose for ethanol production[J]. Carbohydrate Polymers, 2011, 84(3): 865-871.
[48] Chen Y, Sharma-Shivappa R R, Chen C. Ensiling agricultural residues for bioethanol production[J]. Applied Biochemistry Biotechnology, 2007, 143(1): 80-92.
[49] Yukiko S, Hiroko K K. Ethanol production from ensiled rice straw and whole-crop silage by the simultaneous enzymatic saccharification and fermentation process[J]. Bioscience and Bioengineering, 2011, 111(3): 320-325.
[50] Kitamoto H K, Horita M, Cai Y, et al. Silage produces biofuel for local consumption[J]. Biotechnology for Biofuels, 2011, 4(1): 1-10.