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Acta Prataculturae Sinica ›› 2014, Vol. 23 ›› Issue (3): 350-355.DOI: 10.11686/cyxb20140341

• Orginal Article • Previous Articles     Next Articles

Effects of different chemical treatments on fermentation characteristics of rice straw in vitro

MA Yan-yan,LI Yuan-fei,CHENG Yan-fen,ZHU Wei-yun   

  1. Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2013-06-21 Online:2014-06-20 Published:2014-06-20

Abstract: The effects of different chemical treatments on fermentation characteristics of rice straw (RS) in vitro were investigated. The rumen fluid was collected from 3 holstein dairy cows with permanent rumen fistula. The rice straw was treated with composite sodium hydroxide (SH), ammonium bicarbonate (AB) and urea (UR). The bottles containing 10 mL rumen fluid and 90 mL media with 1 g RS as substrate were incubated at 39℃, and the samples were collected at 24, 48, 72 and 96 h after incubation for the analysis of gas production (GP), volatile fatty acids (VFA), ammonia nitrogen (NH3-N) and microbial protein production (MCP). The chemical treatments significantly improved the GP, concentrations of VFA, NH3-N and MCP compared with untreated RS. The cumulative gas production, dry matter digestibility and VFA of SH-RS were significantly higher than the others and they were significantly lower in the untreated RS. However, pH and the concentrations of NH3-N and MCP of UR-RS were significantly higher than those of AB-RS, SH-RS and UT-RS. In conclusion, chemical treatments improved the in vitro digestibility of RS, in which composite sodium hydroxide treatment was recommended.

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