Physiological responses of Pennisetum longissimum var. intermedium seedlings to PEG, low temperature and salt stress treatments

doi:10.11686/cyxb20140222

Abstract

Abstract: The physiological characteristics of Pennisetum longissimum var. intermedium seedlings under three treatments of PEG-6000, low temperature and salt stress were studied using simulation adversity stress methods. The resistance and ecological adaptability were analyzed and comprehensively evaluated: 1) With increased concentrations of PEG the soluble proteins content initially increased but then decreased before increasing again. Soluble sugar initially increased but then decreased and had a maximal value when PEG was 5%. The content of MDA sharply increased at first, then remained stable and finally decreased significantly after a maximal value when PEG was 5%. The activity of SOD sharply decreased at first, then increased slowly then increased significantly. It had a minimal value when PEG was 5% and a maximal value with 20% PEG. 2) The contents of soluble protein and soluble sugar and the activity of SOD increased at first but then decreased with low temperature stress. The content of proline increased with increasing temperatures and MDA also showed an increasing trend. 3) With increased concentrations of salt, the soluble protein content initially increased but then decreased while the contents of soluble sugar, proline and MDA increased. SOD activity showed a slight increase at first, then slowly reduced followed by a rapid decline. Correlation analysis showed that the content of proline was significantly negatively correlated with soluble sugar concentration under PEG treatment. With low temperature stress of 4 hours, the contents of MDA were significantly positively correlated with soluble sugar and proline. With NaCl treatment, the content of soluble sugar was significantly positively correlated with MDA and proline, the activity of SOD was significantly negatively correlated with soluble sugar and MDA while it was significantly positively correlated with soluble protein. To sum up, the physiological dynamic changes of P. longissimum var. intermedium in its response to inverse factor stress reflects its adaptive response of resistance to adversity stresses and reducing injury.

CLC Number:

ZHANG Huai-shan, ZHAO Gui-qi, LI Meng-fei, XIA Zeng-run, WANG Chun-mei. Physiological responses of Pennisetum longissimum var. intermedium seedlings to PEG, low temperature and salt stress treatments[J]. Acta Prataculturae Sinica, 2014, 23(2): 180-188.

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