Abstract: Biosolids contain biologically active substances, such as humus and auxin, which will influence plant growth. The influences of biosolids on drought resistance of tall fescue (Festuca arundinacea) were studied at the same nitrogen level. Split-plot treatments included well-watered and drought stress. Subplot treatments all had nitrogen applied at 75 mg/kg. The control had 75 mg N/kg soil completely provided by NH₄NO₃ solution). The biosolids 0.5× rate had 37.5 mg N/kg soil provided by biosolids, and 37.5 mg N/kg soil provided by NH₄NO₃ solution. The biosolids 1× rate had 75 mg N/kg soil completely provided by biosolids). Nitrogen treatments at biosolids 1× and 0.5× rates improved turfgrass quality, leaf relative water content, leaf chlorophyll content, and reduced leaf wilting rate under drought stress (P<0.05) indicating that biosolids improved the aboveground growth condition of tall fescue under drought stress. Treatment with the biosolids 1× rate also increased leaf water use efficiency of tall fescue and maintained better water metabolism at 50% container capacity (P<0.05). Treatments at biosolids 1× and 0.5×rates increased root dry weight of tall fescue under both moisture conditions and improved root viability under severe drought stress. Proline accumulation was also promoted by bisolids under both water conditions (P<0.05). It is suggested that biologically active substances in biosolids can enhance drought tolerance of tall fescue.