Abstract: Flowering phenology and reproductive features of hybrid offspring are the chief factors that reflect the potentiality of genetic improvement in breeding project. In our study, we investigated the flowering phenology and reproductive features (pollen fertility, seed setting and the process of meiosis in pollen production) of an artificial F₁ population crossed by M. floridulus and M. sacchariflorus. The main results were: 1) The flowering periods of both F₁ population and individual plants are generally long, from the early or mid-June to mid or late-October, individual plants in the population showed high degree of flowering synchrony; the flowering duration of population and single plant can divided into two peaks based on the statistical number of spikelets and tillering during the flowering stage, which appeared at June and September respectively; according to the booting and onset stage, 66.07% individuals were more like the parent species M. sacchariflorus, while other 31.25% were prone to M. floridulus, only 2.68% individuals showed extremely late flowering, these indicated early flowering has dominant heritability; Seed setting rate was negatively correlated with flowering onset, and positively correlated with flowering number and duration. 2) The parent species M. floridulus and M. sacchariflorus had high degree of pollen fertility, while the rate of fertility pollen of F₁ population was obvious higher than of the parents; the pollen fertility of the F₁ population in the second peak is higher than that of the first peak, were (65.42±14.71)% and (50.79±18.61)% respectively; The pollen fertility was significant variant among the F₁ population, the average variant coefficient reach 29.56%; the pollen fertility seemed to with no correction with seeding setting rate. 3)The meiosis process of the pollen mother cell was general normal in the F₁ plants, only small part which less than 2% had chromosome abnormalities. All the above results indicated M. floridulus and M. sacchariflorus have no crossing barrier, enable the potential to produce hybrid cultivars.