Effects of drought stress on lipid peroxidation,osmotic adjustment and activities of protective enzymes in the roots and leaves of orchardgrass

doi:10.11686/cyxb20140316

Abstract

Abstract: To elucidate the mechanism of drought tolerance in orchardgrass, the different responses associated with lipid peroxidation, osmotic adjustment and activity of protective enzymes in the root and leaf to drought stress of two different genotypes of orchardgrass (drought-sensitive “01998” and drought-tolerant “Baoxing”) were investigated. With an extension of drought stress, MDA content and electrolyte leakage in the root and leaf of the two orchardgrasses increased, and total soluble sugar content and free proline content also accumulated. At the same time, soluble protein content, SOD, CAT, POD, APX activities increased initially and then decreased. The accumulation of leaf MDA content and membrane permeability grew fast, indicating that damage on the blades was far more serious than on roots under constant drought stress. Accumulation of osmotic substances, leaf soluble sugar and soluble protein content were higher in shoots than in the roots, while the relative increase of free amino acid was greater in the roots. This might be because the blade is the main area for sugar production, whereas the root system is closely related to the synthesis of amino acids. In addition, APX and POD activities in the roots and leaves of “Baoxing” were higher than those in “01998” under 24 d drought stress, indicating that its ability to scavenge singlet oxygen and H₂O₂ was not weakened by drought stress. Drought tolerant “Baoxing” showed more active and stable peroxidation ability under drought stress.

CLC Number:

S816

JI Yang,ZHANG Xin-quan,PENG Yan,LIANG Xiao-yu,HUANG Lin-kai,MA Xiao,MA Ying-mei. Effects of drought stress on lipid peroxidation,osmotic adjustment and activities of protective enzymes in the roots and leaves of orchardgrass[J]. Acta Prataculturae Sinica, 2014, 23(3): 144-151.

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