Abstract: Cadmium (Cd) toxicity was investigated in Trifolium repens using accumulation ability microstructure physiological. T. repens were treated with Cd²⁺(0, 100, 200, 300, 400, 500 μmol/L) for 7 days. Cd accumulation in different organs of T. repens gradually increased with the increase in the concentration of Cd. The accumulation content of different organs followed the sequence: roots>leaves>stems. Meanwhile, Cd accumulation in the leaves produced certain toxicity: cell walls of mesophyll cell stained deepening, the shape, size, and density were also changed. In response to Cd²⁺, more MDA and ROS contents were produced in leaves than untreated of T. repens. Compared with untreated, the photosynthetic pigments contents decreased significantly (P<0.05). After Cd²⁺ treated, the content of MDA generally increased, and there is a dose-response relationship between the concentration of Cd. Throughout the process of stress, SOD, POD, CAT activity increased initially and then decreased. Correlation analysis showed that: the content of Chl a and Chl b were significantly negatively correlated with Cd²⁺ concentration. While the content of MDA was highly significant positively correlated with Cd²⁺ concentration. The activity of SOD, POD and CAT were highly significant positively among each other. Above three play synergistic role to reduce injury. These physiological indicators reflect T. repens adaptability to Cd²⁺ stress.