Screening, identification and biological function evaluation of endophytic bacteria against potato storage disease

doi:10.11686/cyxb20140332

Abstract

Abstract: Phoma foveata, Colletotrichum coccodes and Fusarium avenaceum are 3 species of potato storage pathogens. The highly resistant bacterial strain 263XY1 tested against potato storage diseases was screened from endophytic bacteria isolated from Kobreasia capillifolia under alpine grassland in the Eastern Qilian Mountains using the confront culture method. The inhibition rates of 263XY1 against F. avenaceum, C. coccodes and P. foveata were 67.17%, 86.17% and 70.89%, respectively. The concentration of IAA secreted by 263XY1 was 3.31 mg/L in the King medium with Trp, and 1.52 mg/L without Trp. The increment of available P was 38.94 mg/L obtained by dissolving inorganic P (phosphate of calcium) and bacterial strain 263XY1 possessed the ability to fix nitrogen. The 16S rDNA sequence analysis indicated that 263XY1 shared 99% homology with Bacillus vallismortis (GenBank accession No. JQ547633.1), so 263XY1 was identified as B. vallismortis.

CLC Number:

S435.32

WANG Ying,WANG Yu-qin,YANG Cheng-de,YAO Yu-ling,CHEN Xiu-rong,XUE Li. Screening, identification and biological function evaluation of endophytic bacteria against potato storage disease[J]. Acta Prataculturae Sinica, 2014, 23(3): 269-275.

References

Reference：
[1]Fu G Ch, Yang w, Song Zh H.Preliminary Discussion on Current Situation of Meadows and Main Reasons of Degradation of China[J]. Inner Mongolia Environmental Sciences, 2009, 21(4): 32-35.
[2]Li Zh H, Xiang J J, Chen Q H, et al. Isolation and Identification of Actinomycete against Fusarium graminearum Schw [J]. Journal of Triticeae Crops, 2007, 27(1): 149-152.
[3]Liu X, Jia J, Atkinson S, et al. Biocontrol potential of an endophytic Serratia sp. G3 and its mode of action[J]. World Journal of Microbiology and Biotechnology, 2010, 26: 1465-1471.
[4]Zhu F, Chen X J, Tong Y H. Isolation of Rice Endophytic Bacteria and Their Antagonism and Potential Pathogenicity[J]. Chinese Journal of Biological Control, 2007, 23(1): 68-72.
[5]Sessitsch A, Reiter B, Berg G. Endophytic bacterial communities of field-grown-potato plants and their plant-growth-promoting and antagonistic abilities[J]. Canadian Journal of Microbiology, 2004, 50(4): 239-249.
[6]Wang M Q, Liu H P, Han J C, et al. Population Dynamics of Endophytic Bacteria in Tomato Plant and Screening of Antagonistic Strains[J]. Chinese Agricultural Science Bulletin, 2010, 26(9): 277-282.
[7]Liu Q L, Zhang J X, Xu R F, et al. Studies on Isolation of Endophytic Bacteria from Citrus Skins and Screening of Antagonistic Strains Against Penicillium italicum[J]. Chinese Agricultural Science Bulletin, 2011, 27(28): 235-239.
[8]Yi L, Ma G H, Xiao C G. Inhibition effect and screening of antagonistic endophytic bacteria against Thielaviopsis basicola[J]. Microbiology, 2012, 39(10): 1464-1470.
[9]Zhang S M, Sha C Q, Wang Y X, et al. Isolation and characterization of antifungal endophytic bacteria from soybean[J]. Microbiology, 2008, 35(10): 1593-1599.
[10]li Ch H,Deng Y Y, Zhao M W. Population dynamics and antagonism toward Fusarium oxysporium f. Sp.Vasinfectum. And Verticillium dahliae Kleb of endophytic bacteria from cotton[J]. Acta Microbiologica Sinica, 2009, 49(9): 1196-1202.
[11]Song G T, Zhou G Y, Liu J A. Isolation and characterization of antagonistic endophytic bacteria again Fusarium proliferatum[J]. Acta Phytophylacica Sinica, 2010, 37(2): 137-142.
[12]Hinton D M, Bacon C W. Enterobacter cluavae is an endophytic symbiont of corn[J]. Mycopathologia, 1995, 129: 117-125.
[13]Xia Z J, Gu B K, Wu A M, et al. Isoenzymatic activities involved in induced resistance of cotton to vert-cillium dahliae by endophytic bacteria[J]. Jiangsu Journal of Agricultural Sciences, 1997, 13: 99-101.
[14]Zhang J H, Wang C L, Guo S X, et al. Studies on the plant hormones produced by 5 species of endophytic fungi isolated from medicinal plants(or chidace a)[J]. Acta Academiae Medicinae Sinicae, 1999, 21(6): 460-465.
[15]Rana A, Saharan B, Joshi M. Identification of multi trait PGPR isolates and evaluating their potential as inoculants for wheat[J]. Annals of Microbiology, 2011, 61: 893-900.
[16]Prasad A, Kumar S, Pandey A, et al. Microbial and chemical sources of phosphorus supply modulate the yield and chemical composition of essential oil of rose-scented geranium (Pelargonium species) in sodic soil[J]. Biology and Fertility of Soils, 2012, 48: 117-122.
[17]Karagoz K, Ates F, Karagoz H. Characterization of plant growth-promoting traits of bacteria isolated from the rhizosphere of grapevine grown in alkaline acidic soils[J]. European Journal of Soil Biology, 2012, 50: 144-150.
[18]Lin L, Guo W, Xing Y X. The actinobacterium Microbacterium sp. 16SH accepts pBBR1-based pPROBE vectors, forms biofilms, invades roots，and fixes N2 associated with micropropagated sugarcane plants[J]. Applied Microbiology and Biotechnology, 2012, 93(3): 1185-1195.
[19]Jiang H X, Yang Ch D, Xue L, et al. Identification and biological characteristics of the pathogen causing the potato gangrene in Gansu Province[J]. Acta Prataculturae Sinica, 2013, 22(2): 123-131.
[20]Yang Ch D, Jiang H X, Chen X R, et al. Identification of potato black dot disease caused by Colletotrichum coccodes and screening for fungicides in the laboratory in Gansu Province[J]. Plant Protection, 2012, 38(6): 127-133.
[21]Xue Y F, Meng M L,Hu J, et al. Determination for toxicity of six fungicides to fusarium wilt pathogen of potato in vitro[J]. Chinese Potato Journal, 2012, 26(4): 228-230.
[22]Fang Z D. Plant Pathology Research Methods (third edition)[M]. Beijing: China Agriculture Press, 1997.
[23]Lin Q M, Zhao X R, Sun Y X.Community characters of soii phosphobacteria in four ecosystems[J]. Ecology and Environmental Sciences, 2000, 9(1): 34-37.
[24]Zhao B, He S J. Microbiology[M]. Beijing: Science Press, 2002.
[25]Glickmann E, Dessaux Y. A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria[J]. Applied and Environmental Microbiology, 1995, 619(2): 793-796.
[26]Li Z D, Chen X R, Li P, et al. Identification of Polygonum viviparum endophytic bacteria Z5 and determination of the capacity to secrete IAA and antagonistic capacity towards pathogenic fungi[J]. Acta Prataculturae Sinica,, 2010, 19(2): 61-68.
[27]Shi R H. Agrochemical soil analysis[M]. Beijing: Agriculture Press, 1981.
[28]Wang J B, Zjang D G, Cao G M, et al. Regional characteristics of the alpine meadow degradation succession on the Qinghai-Tibetan Plateau[J]. Acta Prataculturae Sinica, 2013, 22(2): 1-10.
[29]Li Y Y, Dong Sh K, Zhu L, et al. Adaptation strategies of reproduction of plant community in response to grassland degradation and artificial restoration[J]. Acta Ecologica Sinica, 2013, 33(15):4683-4691.
[30]Lu C L, Xie G D, Cheng S K, et al. Rangeland resources utilization of china conflict and coordination between product function and ecological function[J]. Journal of Natural Resources,, 2009, 24(10): 1685-1693.
[31]Xu Y Y, Du B, Hai Y L, et al. Diversity of antagonistic bacteria isolated from rhizosphere of several cash crops[J].Chinese Journal of Applied Ecology, 2012, 23(2): 511-518.
[32]Regupathy T V, Young J Y, Sun H L, et al. Diversity of endophytic bacteria in ginseng and their potential for plant growth promotion[J]. The Journal of Microbiology, 2010, 48(5): 559-565.
[33]Bacon C W, Hinton D M. Endophytic and biological control potential of Bacillus mojavensisand related species[J]. Biological Control, 2002, 23(3): 274-284.
[34]Qian G L, Hu B S, Jiang Y H, et al. Identification and characterization of Lysobacter enzymogenesas a biological control agent against some fungal pathogens[J]. Agricultural Sciences in China, 2009, 8: 68-75.
[35]Zhou J Y, J Y, Wang H W, et al. Diversity and plant growth-promoting potential of culturable endophytic bacteria isolated from the leaves of Atractylodes lancea[J]. Acta Ecologica Sinica, 2013, 33(4): 1106-1117.
[36]Zhang Y, Zhu Y, Yao T, et al. Interactions of four PGPRs isolated from pasture rhizosphere[J]. Acta Prataculturae Sinica, 2013, 22(1): 27-37.
[37]Gao X X, Man B Y, Chen X R, et al. Identification and determination of biological characteristics of Kobresia capillifolia endophytic bacteria X4 in the East Qilian Mountain Alpine grasslands[J]. Acta Prataculturae Sinica, 2013, 22(4): 137-146.
参考文献：
[1]付国臣, 杨韫, 宋振宏. 我国草地现状及其退化的主要原因[J]. 内蒙古环境科学, 2009, 21(4): 32-35.
[2]李正辉, 向晶晶, 陈婧鸿, 等. 小麦赤霉病拮抗菌的分离与鉴定[J]. 麦类作物学报, 2007, 27(1): 149-152.
[3]Liu X, Jia J, Atkinson S,et al. Biocontrol potential of an endophytic Serratia sp. G3 and its mode of action[J]. World Journal of Microbiology and Biotechnology, 2010, 26: 1465-1471.
[4]朱凤, 陈夕军, 童蕴慧. 水稻内生细菌的分离及其拮抗性与潜在致病性测定[J]. 中国生物防治, 2007, 23(1): 68-72.
[5]Sessitsch A, Reiter B, Berg G. Endophytic bacterial communities of field-grown-potato plants and their plant-growth-promoting and antagonistic abilities[J]. Canadian Journal of Microbiology, 2004, 50(4): 239-249.
[6]王美琴, 刘慧平, 韩巨才, 等. 番茄内生细菌种群动态分析及拮抗菌株的筛选[J]. 中国农学通报, 2010, 26(9): 277-282.
[7]刘起丽, 张建新, 徐瑞富, 等. 柑橘皮内生细菌分离及柑橘青霉病菌拮抗菌筛选研究[J]. 中国农学通报, 2011, 27(28): 235-239.
[8]易龙, 马冠华, 肖崇刚. 烟草根黑腐病拮抗内生细菌的筛选及其抑菌作用[J]. 微生物学通报, 2012, 39(10): 1464-1470.
[9]Zhang S M, Sha C Q, Wang Y X,et al. Isolation and characterization of antifungal endophytic bacteria from soybean[J]. Microbiology, 2008, 35(10): 1593-1599.
[10]李春宏, 邓渊钰, 赵明文. 棉花内生细菌数量动态及其对棉花黄、枯萎病菌的拮抗作用[J]. 微生物学报, 2009, 49(9): 1196-1202.
[11]宋光桃, 周国英, 刘君昂. 油茶根腐病拮抗内生细菌的筛选及鉴定[J]. 植物保护学报, 2010, 37(2): 137-142.
[12]Hinton D M, Bacon C W. Enterobacter cluavae is an endophytic symbiont of corn[J]. Mycopathologia, 1995, 129: 117-125.
[13]夏正俊, 顾本康, 吴蔼民, 等. 棉株植物内生菌诱导棉花抗黄萎病过程中同功酶活性的变化[J]. 江苏农业学报, 1997, 13: 99-101.
[14]张集慧, 王春兰, 郭顺星, 等. 兰科药用植物的5种内生菌产生的植物激素[J]. 中国医学科学院学报, 1999, 21(6): 460-465.
[15]Rana A, Saharan B, Joshi M. Identification of multi-trait PGPR isolates and evaluating their potential as inoculants for wheat[J]. Annals of Microbiology, 2011, 61: 893-900.
[16]Prasad A, Kumar S, Pandey A,et al. Microbial and chemical sources of phosphorus supply modulate the yield and chemical composition of essential oil of rose-scented geranium (Pelargonium species) in sodic soil[J]. Biology and Fertility of Soils, 2012, 48: 117-122.
[17]Karagoz K, Ates F, Karagoz H. Characterization of plant growth-promoting traits of bacteria isolated from the rhizosphere of grapevine grown in alkaline acidic soils[J]. European Journal of Soil Biology, 2012, 50: 144-150.
[18]Lin L, Guo W, Xing Y X. The actinobacterium Microbacterium sp. 16SH accepts pBBR1-based pPROBE vectors, forms biofilms, invades roots，and fixes N2 associated with micropropagated sugarcane plants[J]. Applied Microbiology and Biotechnology, 2012, 93(3): 1185-1195.
[19]姜红霞, 杨成德, 薛莉, 等. 甘肃省马铃薯坏疽病鉴定及其病原生物学特性研究[J]. 草业学报, 2013, 22(2): 123-131.
[20]杨成德, 姜红霞, 陈秀蓉, 等. 甘肃省马铃薯炭疽病的鉴定及室内药剂筛选[J]. 植物保护, 2012, 38(6): 127-133.
[21]薛玉凤, 蒙美莲, 胡俊, 等. 6 种杀菌剂对马铃薯枯萎病菌的室内毒力测定[J]. 中国马铃薯, 2012, 26(4): 228-230.
[22]方中达. 植病研究方法(第三版)[M]. 北京: 中国农业出版社, 1997.
[23]林启美, 赵小蓉, 孙炎鑫. 四种不同生态系统中的解磷细菌数量及种群分布[J]. 土壤与环境, 2000, 9(1): 34-37.
[24]赵斌, 何绍江. 微生物学实验[M]. 北京: 科学出版社, 2002.
[25]Glickmann E, Dessaux Y. A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria[J]. Applied and Environmental Microbiology, 1995, 619(2): 793-796.
[26]李振东, 陈秀蓉, 李鹏, 等. 珠芽蓼内生菌Z5产吲哚乙酸和抑菌能力测定及其鉴定[J]. 草业学报, 2010, 19(2): 61-68.
[27]史瑞和. 土壤农化分析[M]. 北京: 农业出版社, 1981.
[28]王建兵, 张德罡, 曹广民, 等. 青藏高原高寒草甸退化演替的分区特征[J]. 草业学报, 2013, 22(2): 1-10.
[29]李媛媛, 董世魁, 朱磊, 等. 青藏高原高寒草甸退化与人工恢复过程中植物群落的繁殖适应对策[J]. 生态学报, 2013,33(15); 4683-4691.
[30]鲁春霞, 谢高地, 成升魁, 等. 中国草地资源利用: 生产功能与生态功能的冲突与协调[J]. 自然资源学报, 2009, 24(10): 1685-1693.
[31]徐莹莹, 杜秉, 海姚良, 等. 同几种经济作物根际拮抗细菌的多样性[J]. 应用生态学报, 2012, 23(2): 511-518.
[32]Regupathy T V, Young J Y, Sun H L,et al. Diversity of endophytic bacteria in ginseng and their potential for plant growth promotion[J]. The Journal of Microbiology, 2010, 48(5): 559-565.
[33]Bacon C W, Hinton D M. Endophytic and biological control potential of Bacillus mojavensis and related species[J]. Biological Control, 2002, 23(3): 274-284.
[34]Qian G L, Hu B S, Jiang Y H,et al. Identification and characterization of Lysobacter enzymogenes as a biological control agent against some fungal pathogens[J]. Agricultural Sciences in China, 2009, 8: 68-75.
[35]周佳宇, 贾永, 王宏伟, 等. 茅苍术叶片可培养内生细菌多样性及其促生潜力[J]. 生态学报, 2013, 33(4): 1106-1117.
[36]张英, 朱颖, 姚拓, 等. 分离自牧草根际四株促生菌株（PGPR）互作效应研究[J]. 草业学报, 2013, 22(1): 27-37.
[37]高晓星, 满百膺, 陈秀蓉, 等. 东祁连山线叶嵩草内生细菌X4的产吲哚乙酸、解磷、抗菌和耐盐特性研究及分子鉴定[J]. 草业学报, 2013, 22(4): 137-146.