Identification and evaluation of biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands

doi:10.11686/cyxb20140334

Abstract

Abstract: To study the biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands, the determination of bacteriostatic ability was carried out by dual culture against plant pathogenic fungi. B-401 inhibited the growth of Phoma foveata, Stysanus stemonitis, Colletotrichum coccodes, Fusarium avenaceum, Fusarium oxysporum, Alternaria solani, Bipolaris sorokiniana and Fusarium solani by 74.45%, 71.57%, 70.05%, 48.01%, 56.01%, 60.25%, 64.65% and 45.01% respectively. The control efficacy of B-401 against potato gangrene was 52.67% when a 10 times dilution of fermentation broth was sprayed on potato tubers during the storage-period. The concentration of IAA secreted by B-401 in King’s medium containing 100 mg/L tryptophan was 3.42 mg/L, and the concentration in the medium without tryptophan was 2.60 mg/L. B-401 possessed the capacity for nitrogen fixation but it did not have the capacity for phosphate solubilization. Based on morphology and physiological and biochemical characters, combined with 16S rDNA gene sequence analysis, B-401 was indentified as Bacillus atrophaeus.

CLC Number:

S812.29

CHANG Tao,WANG Han-qi,YANG Cheng-de,WANG Ying,YANG Xiao-li,XUE Li,CHEN Xiu-rong,XU Chang-lin. Identification and evaluation of biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands[J]. Acta Prataculturae Sinica, 2014, 23(3): 282-289.

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