solation of anaerobic fungi from the rumen and faeces of goat and comparison of fermentation activity between isolates in vitro

doi:10.11686/cyxb20140443

Abstract

Abstract:

The anaerobic fungi in rumen fluid and faeces of two local goats in Nanjing area were isolated. The genera Neocallimastix, Piromyces, Orpinomyces and Anaeromyces were isolated from the rumen liquid of goat, and the genera Neocallimastix and Piromyces were isolated from the fresh faeces. The ability to degrade rice straw, wheat straw and cornstalk by these isolates was compared. The degradability of rice straw by all isolates was greater than 30%, while that of corn straw was less than 25%. When compared with the isolates from rumen liquid, the degradation by species isolated from faeces had a lower total gas production and degradability of dry matter when the three forages were used as substrates. There were some differences in degradability of forage by these organisms which could be explained by the differences in composition of the three substrates and by the characteristics of these isolates.

CLC Number:

S827

MAO Sheng-yong, YAO Wen, CHEN Yong. solation of anaerobic fungi from the rumen and faeces of goat and comparison of fermentation activity between isolates in vitro[J]. Acta Prataculturae Sinica, DOI: 10.11686/cyxb20140443.

References

Reference：
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[4]Borneman W S, Hartley R D, Morrison W H, et al. Feruloyl and P coumaroyl esterase from anaerobic fungi in relation to plant cell wall degradation[J]. Applied Microbiology and Biotechnology, 1990, 33: 345-351.
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[8]Davies R D, Theodorou M K, Lawrence M I G. Distribution of anaerobic fungi in the digestive tract of cattle and their survival in faeces[J]. Journal of General Microbiology, 1993, 139: 1395-1400.
[9]Theodorou M K, Davies R D, Jordan C G, et al. Comparision of anaerobic fungi in faeces and rumen digesta of newly born and adult ruminants[J]. Mycology Research, 1993, 97(10): 1245-1252.
[10]Orpin C G, Joblin K N. The Rumen Microbial Ecosystem (2nd ed.)[M]. London: Elsevier Applied Science Press, 1988.
[11]Ho Y W, Abdullah N, Jalaludin S. Piromyces spiralis, a new species of anaerobic fjungus from the rumen of goat[J]. Mycotaxon, 1993, 48: 59-68.
[12]Borneman W S, Akin D E, Ljungdahl L G. Fermentation products and plant cell wall degrading enzymes produced by monocentric and polycentric anaerobic ruminal fungi[J]. Applied and Environmental Microbiology, 1989, 55: 1066-1073.
[13]Ho Y W, Wong N, Abdullah H, et al. Fermentation activities of some new species of anaerobic rumen fungi from Malaysia[J]. Journal of General Microbiology, 1996, 42: 51-59.
[14]William A G, Orpin C G. Polyxaccharide degrading enzymes formed by three species of anaerobic rumen fungi grow on a range of carbohydrate substrates[J]. Canadian Journal of Microbiology, 1987, 33(5): 418-426.

参考文献：
[1]Orpin C G. Studies on the rumen flagellate Neocallimastix frontalis[J]. Journal of General Microbiology, 1975, 91: 249-262.
[2]Ho W Y, Barr S J D. Classification of anaerobic gut fungi from herbivored with emphasis on rumen fungi from Malaysia[J]. Mycologia, 1995, 87(5): 655-677.
[3]Akin D E, Borneman W S, Lyon C E. Degradation of leaf blades and stems by monocentric and polycentric isolates of ruminal fungi[J]. Animal Feed Science and Technology, 1995, 31: 205-221.
[4]Borneman W S, Hartley R D, Morrison W H, et al. Feruloyl and P-coumaroyl esterase from anaerobic fungi in relation to plant cell wall degradation[J]. Applied Microbiology and Biotechnology, 1990, 33: 345-351.
[5]Zhu W Y. Growth and survival of anaerobic fungi in batch culture and continuous-flow culture[J]. Anaerobe, 1996, 2: 29-37.
[6]艾丽霞, 苏勇, 朱伟云. 梅山与长白母猪粪样微生物体外发酵八种纤维底物的特性比较[J]. 草业学报, 2013, 22(3): 99-107. 
[7]Theodorou M K, Gill M, King-Spooner C, et al. Enumeration of anaerobic chytridiomycetes as thallus forming units: a novel method for the quantification of fibrolytic fungal populations from the digestive tract ecosystem[J]. Applied and Environmental Microbiology, 1990, 56: 1073-1078.
[8]Davies R D, Theodorou M K, Lawrence M I G. Distribution of anaerobic fungi in the digestive tract of cattle and their survival in faeces[J]. Journal of General Microbiology, 1993, 139: 1395-1400.
[9]Theodorou M K, Davies R D, Jordan C G, et al. Comparision of anaerobic fungi in faeces and rumen digesta of newly born and adult ruminants[J]. Mycology Research, 1993, 97(10): 1245-1252.
[10]Orpin C G, Joblin K N. The Rumen Microbial Ecosystem (2nd ed.)[M]. London: Elsevier Applied Science Press, 1988.
[11]Ho Y W, Abdullah N, Jalaludin S. Piromyces spiralis, a new species of anaerobic fjungus from the rumen of goat[J]. Mycotaxon, 1993, 48: 59-68.
[12]Borneman W S, Akin D E, Ljungdahl L G. Fermentation products and plant cell wall degrading enzymes produced by monocentric and polycentric anaerobic ruminal fungi[J]. Applied and Environmental Microbiology, 1989, 55: 1066-1073.
[13]Ho Y W, Wong N, Abdullah H, et al. Fermentation activities of some new species of anaerobic rumen fungi from Malaysia[J]. Journal of General Microbiology, 1996, 42: 51-59.
[14]William A G, Orpin C G. Polyxaccharide degrading enzymes formed by three species of anaerobic rumen fungi grow on a range of carbohydrate substrates[J]. Canadian Journal of Microbiology, 1987, 33(5): 418-426.