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Acta Prataculturae Sinica ›› 2014, Vol. 23 ›› Issue (5): 175-183.DOI: 10.11686/cyxb20140520

• Orginal Article • Previous Articles     Next Articles

Effects of Na+ on antioxidant defence and accumulation of osmoregulatory substances in white clover under water deficit stress

LI Zhou,WANG Xiao-juan,PENG Dan-dan,PENG Yan   

  1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China
  • Received:2013-09-17 Online:2014-10-20 Published:2014-10-20

Abstract:

Effects of NaCl on relative water content, root viability, chlorophyll content, reactive oxygen species, relative membrane permeability and membrane lipid peroxidation, antioxidant defence and accumulation of osmoregulatory substances in white clover (Trifolium repens) under water stress induced by PEG-6000 were analyzed. Under water stress, exogenous NaCl significantly improved relative water content and root viability, and effectively reduced the decomposition of chlorophyll in leaves. The treatment of exogenous NaCl improved SOD, POD, CAT activities and the key enzymes of AsA-GSH cycle activities and maintained significantly lower electrolyte leakage and MDA content, thereby reducing water deficit stress injury to plants. 50 mmol/L NaCl also can improve the growth of white clover under normal water availability, but the accumulation of soluble sugar and free proline was significantly lower in the exogenous NaCl treatment under water deficit stress. The results suggested that exogenous NaCl can effectively improve water deficit stress tolerance of white clover with mechanisms including enhanced antioxidant defence, but not accumulation of more osmoregulatory substances. The reason maybe that white clover is like other xerophytes which accumulate Na+ as an osmoregulatory solute under drought stress.

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