Identification and determination of biological functions of endophytic bacteria from Achnatherum inebrians in Alpine grassland of East Qilian Mountains

doi:10.11686/cyxb20140529

Abstract

Abstract:

6 endophytic bacteria of Achnatherum inebrians collected from Alpine grassland on East Qilian Mountains were isolated using a surface disinfection method, and named 261MG1, 261MG2, 261MG3, 261MG4, 261MY5 and 261MY6, respectively. Of these, 5 strains had strong antifungal activity against Colletotrichum coccodes and Phoma foveata, and 4 strains were active against Fusarium avenaceum. In addition, 261MG2, 261MG3, 261MG4 and 261MY6 had inhibitory activity against 3 other pathogens. Except for 261MG4, the endophytic bacteria had the nitrogen-fixing ability and IAA (indole acetic acid) secreting. Based on the culture characteristics, morphological characteristics and 16S rDNA gene sequence analysis, 261MG1, 261MG2, 261MG3, 261MY6, 261MG4 and 261MY5 were identified as Bacillus subtilis, Bacillus axarquiensis, Bacillus mojavensis, Bacillus amyloliquefaciens, Brevibacterium michiganensis, and Clavibacter sp., respectively. The study provides the evidence for the existence of endophytic bacteria of A. inebrians in East Qilian Mountains Alpine grassland, and the initial exploration of their identity and properties.

CLC Number:

S543⁺.901

YANG Cheng-de,WANG Ying,WANG Yu-qin,YAO Yu-ling,XUE Li,XU Chang-lin,CHEN Xiu-rong. Identification and determination of biological functions of endophytic bacteria from Achnatherum inebrians in Alpine grassland of East Qilian Mountains[J]. Acta Prataculturae Sinica, 2014, 23(5): 249-255.

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