The genetic variation revealed by SSR markers in crossbreeding groups of annual ryegrass after continuous mass selection

doi:10.11686/cyxb20140541

Abstract

Abstract:

SSR markers were used to evaluate the genetic diversity in two crossbreeding groups of annual ryegrass after 5 cycles mass selection. With the 20 primer pairs selected, a total of 217 bands were detected in the two populations and the percentage of polymorphic bands was 92.2%. In the two crossbreeding groups, 72.3% or 75.2% of the genetic diversities were estimated intra-populations and 27.7% or 24.8%, respectively, were estimated inter-populations. The genetic diversity of the inter-population was much more than that of the intra-population. After analysis of the bands, it was found from the amplified results that the crossbreeding groups had more bands shared by parents and progeny but fewer non parental specific bands. As mass selection proceeded in the crossbreeding groups, the percentage of polymorphic bands in populations declined as did the Shannon index and Nei’s gene diversity. It is shown that genetic diversity of each population declined under mass selection. The results of this study will provide some basic information for ryegrass crossbreeding.

CLC Number:

S543⁺.603.51

WANG Shao-fei,HUANG Lin-kai,ZHANG Xin-quan,MA Xiao. The genetic variation revealed by SSR markers in crossbreeding groups of annual ryegrass after continuous mass selection[J]. Acta Prataculturae Sinica, 2014, 23(5): 345-351.

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