Genetic diversity and phylogeny of rhizobia isolated from white clover in Sichuan Province

doi:10.11686/cyxb20140516

Abstract

Abstract:

The objective of this study was to determine the genetic diversity and phylogeny of 69 isolates of symbiotic bacteria associated with wild white clover obtained from six sites in Sichuan Province, China: Ya’an, Kangding, Luding, Xichang, Chengdu and Leshan. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and phylogenetic analyses of the 16S rDNA gene, housekeeping genes (recA, glnII and atpD), nodulation gene (nodC) and nitrogen-fixing gene (nifH) were conducted in the research. Four PCR-RFLP fingerprint patterns were identified among the 69 isolates from the 16S rDNA analysis, indicating relatively high genetic diversity. The results of phylogenetic analysis of 16S rDNA and housekeeping genes were similar; nine representative strains mainly belonged to the genus Rhizobium in Alpha-Proteobacteria were identified. In addition, nodC and nifH genes from strains from the Rhizobium genus were successfully amplified by PCR, but strains belonging to the Agrobacterium genus were not able to be amplified. All of the isolates could be identified by species with sixty-eight strains confirmed as white clover rhizobia. The diversity of white clover rhizobia differed at different sites, indicating the potential to increase the availability of beneficial rhizobia strains.

CLC Number:

S816

PAN Ming-hong,LING Yao,JING Wen,MA Hong-ping,PENG Yan. Genetic diversity and phylogeny of rhizobia isolated from white clover in Sichuan Province[J]. Acta Prataculturae Sinica, 2014, 23(5): 143-152.

References

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