Preliminary studies on transgenic chicory using the sulphur-amino acid gene, γ-zein, mediated by Agrobacterium tumefacien

doi:10.11686/cyxb2014307

Abstract

Abstract: Sulfur-containing amino acids have important physiological functions related to animal nutrition and immunity. To improve the sulfur-amino acid content of chicory, leaves of chicory were transformed with the Sulphur-amino acid gene γ-zein, an important prolamin storage protein from Zea mays and a green fluorescent protein (GFP) gene using Agrobacterium mediated transfusion. After co-culture, selective differentiation and regeneration, hygromycin resistant plants were obtained. Resistant plants were detected using PCR, PCR-southern, dot blot hybridization and RT-PCR. The results demonstrated that the γ-zein genes had been integrated into the genome of chicory and expressed on a nucleic acid level in the transgenic plants.

ZHANG Yu, BAI Shi-Qie, LI Cong. Preliminary studies on transgenic chicory using the sulphur-amino acid gene, γ-zein, mediated by Agrobacterium tumefacien[J]. Acta Prataculturae Sinica, 2015, 24(9): 73-79.

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