An evaluation of the resistance of alfalfa cultivars to Stemphylium and Phoma leaf spot diseases

doi:10.11686/cyxb2014337

Abstract

Abstract: Forty alfalfa (Medicago sativa) cultivars were collected from China and abroad and inoculated with suspensions of different concentrations of spores from Stemphylium leaf spot and Phoma leaf spot diseases to evaluate the resistance of alfalfa to these two leaf spot diseases and identify the optimal inoculation concentration. The disease indices of 10 cultivars inoculated with these two fungi gradually increased with concentration increase of the spore suspensions. The appropriate inoculation concentrations for comparing cultivar resistance to Stemphylium and Phoma were 1×10⁵ to 1×10⁶ spores per milliliter and 1×10⁶ spores per milliliter, respectively. With a concentration of 1×10⁶ spores per milliliter in the inoculate, the disease indices of Stemphylium leaf spot in forty cultivars ranged from 3.33 to 35.83, while the disease indices of Phoma leaf spot ranged from 13.07 to 52.27. There were significant differences between the 40 cultivars (P<0.05). Fourteen cultivars were found to be resistant to Stemphylium leaf spot, including one highly-resistant cultivar-Gongnong No.2 and thirteen moderately-resistant cultivars. Only one cultivar, Rambler, from America was found to have moderate resistance to Phoma leaf spot.

WANG Yu, LIU Yi, ZHOU Bin-Bin, YUAN Qing-Hua, ZHANG Li, PAN Long-Qi. An evaluation of the resistance of alfalfa cultivars to Stemphylium and Phoma leaf spot diseases[J]. Acta Prataculturae Sinica, 2015, 24(7): 155-162.

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