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    20 July 2015, Volume 24 Issue 7
    Simulating of the response of soil heterotrophic respiration to climate change and nitrogen deposition in alpine meadows
    LI Dong, LUO Xu-Peng, CAO Guang-Min, WU Qin, ZHUO Ma-Cuo, LI Hui-Mei, YANG Yong-Mei, PANG Bing-Kun
    2015, 24(7):  1-11.  DOI: 10.11686/cyxb2014297
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    Vegetation, soil and climate data in the study area was used to simulate the CO2 flux from soil heterotrophic respiration in alpine meadows using the CENTURY model. The results showed that the CENTURY model was able to predict seasonal changes in soil heterotrophic respiration which closely matched observed changes. The linear regression equation between observed and simulation values at Fengxiakou and Ganchaitan were y=0.7776x+23.796 (R2=0.6885, n=31) and y=0.9487x-8.6994 (R2=0.6062, n=30), respectively. In the past 46 years (1960-2005), annual average temperature in the study area increased at a rate of 0.35℃/decade. Precipitation has remained with relatively stable. Simulation of soil heterotrophic respiration CO2 flux in alpine meadows using CENTURY over this period revealed a slowly rising trend (16.5 g C/m2·decade) ranging between 479.2-624.9 g C/(m2·a). Correlation analysis between temperature and rainfall showed that soil heterotrophic respiration CO2 flux had a significant positive correlation with temperature (r=0.70, P<0.05) but was not correlated with precipitation. Nitrogen application significantly promoted soil heterotrophic respiration CO2 flux. Medium nitrogen (MN) and high nitrogen (HN) showed highly significant increases compared to the control (zero nitrogen) (P<0.01), but there was no difference between MN and HN. The results suggest that alpine meadow limited by low temperature and nitrogen was sensitive to climate change.
    Effect of lucerne-crop rotations on soil physical properties in the semiarid Loess Plateau of Central Gansu
    SONG Li-Ping, LUO Zhu-Zhu, LI Ling-Ling, CAI Li-Qun, ZHANG Ren-Zhi, NIU Yi-Ning
    2015, 24(7):  12-20.  DOI: 10.11686/cyxb2015009
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    A long-term field experiment comparing the effects of different lucerne-crop rotations on soil physical properties was conducted at Dingxi, western Loess Plateau. Six rotation patterns [lucerne-lucerne (L-L), lucerne-fallow (L-F), lucerne-wheat (L-W), lucerne-corn (L-C), lucerne-potato (L-P) and lucerne-millet (L-M)] were compared. There were significant differences in soil bulk density, soil total porosity, soil saturated hydraulic conductivity, soil sorptivity, macroscopic capillary length and mean pore diameter among the six rotation patterns, but soil aggregate stability was not affected. Compared with those under L-L, soil bulk density under L-F, L-W, L-C, L-P and L-M was reduced by 6.86%, 7.69%, 6.22%, 7.69% and 6.22% respectively, soil sorptivity increased by 1.19, 2.31, 0.25, 0.66 and 0.75 times respectively, macroscopic capillary length increased by 3.73, 2.92, 1.49, 2.62 and 1.15 times respectively and mean pore diameter reduced by 79.43%, 72.99%, 79.43%, 69.41% and 56.35% respectively. Compared with L-L, soil saturated hydraulic conductivity under L-F,L-W and L-M increased by 0.32, 1.52 and 0.33 times, respectively. The effect of rotation on soil aggregate mainly influenced the 0-10 and 10-30 cm soil layers. It was concluded that lucerne-crop rotations improved soil structure and permeability, improving soil physical quality in the semi-arid areas on the Loess Plateau.
    Allelopathy and the rhizospere bacterial community structure of Oxytropis ochrocephala
    HE Wei, GUO Lin-Wei, FAN Peng-Hui, GUO Bin, FU Yan-Ping, WEI Ya-Hui
    2015, 24(7):  21-29.  DOI: 10.11686/cyxb2014504
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    Oxytropis ochrocephala, a toxic perennial legume grass, is widely distributed across the grasslands of western China. Developing effective control methods for O. ochrocephala requires exploration of mechanisms contributing to its occurrence. Allelopathy is considered to be an underlying mechanism driving biological invasion in many plant species. In this study, the allelopathic effect of O. ochrocephala on the growth of Medicago sativa and Lolium perenne was analyzed, and the soil bacterial community from the rhizosphere and non-rhizosphere soils of O. ochrocephala were compared to examine if the plants exert allelopathy by altering soil bacterial communities. We planted Medicago sativa and Lolium perenne with soil containing different concentrations of O. ochrocephala tissue powder. After growing for 60 d, plant height, biomass and chlorophyll content were measured. The results showed strong allelopathic effects on the growth of the two grass species with soil containing 5% O. ochrocephala tissue powder, in which plant height, biomass and chlorophyll content were significantly reduced. We compared the bacterial structure of O. ochrocephala rhizosphere and non-rhizosphere soils with 16S rDNA sequencing. The data showed that the structure of the bacterial communities differed greatly and the percentage of some phyla, such as Proteobacteria, Actinobacteria and Acidobacteria, were significantly different. In addition, there were more OTUs (Operational Taxonomic Units) in the non-rhizosphere soil. Since bacteria from different phyla are considered to function discriminately, these changes mean it is highly likely that O. ochrocephala implements allelopathy by altering the structure of the rhizospere bacterial community, and accordingly promotes its growth and competes with other associated plants. These results will assist the development of controlling strategies for O. ochrocephala in the future.
    A comprehensive evaluation of the wild ground cover plants resources in Yunshan, Hunan
    WANG Ye-She, CHEN Li-Jun, YANG Xian-Jun, DUAN Lin-Dong
    2015, 24(7):  30-40.  DOI: 10.11686/cyxb2014344
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    In order to fully understand and potentially utilize the wild ground cover plant resources in Yunshan, Hunan, a systemic analysis and evaluation of wild ground cover resources was undertaken. A combination of field investigation and verification methods, and an analytic hierarchy process (AHP) was used. The results show that 498 wild ground cover species were found in Yunshan, belonging to 107 families and 254 genera. These plants include 14 families, 20 genera and 30 species of pteridophyte; 80 families, 194 genera and 406 dicotyledonous species; 13 families, 40 genera and 62 species of monocotyledonous plants; no gymnosperms were found. Herbs were the dominant vegetation form found in this study, comprising 201 species, 40.36% of the total. Lianas, shrubs, ferns and bamboos were also found, accounting for 29.52%, 23.49%, 6.02% and 0.62% of the total species, respectively. The proportion of erect, creeping and climbing plants differed, accounting for 59.84%, 29.52% and 10.64% respectively. Most of the wild ground cover plants have good ornamental characteristics, good adaptability and strong resistance. Thirty potentially high (Grade I) value ground cover plants were identified in the study. A further 82 second grade and 103 third grade ground cover species were also identified. The wild ground cover plants resources in Yunshan, Hunan were found to be abundant with many species having excellent ground cover features which could be developed for commercial utilization.
    Phenotypic variation and covariation in natural populations of the exotic weed Gaura parviflora in different habitat
    LIU Long-Chang, DU Gai-Gai, SI Wei-Jie, WANG Fei, LUO Hai-Jun, ZHOU Zheng-Jun
    2015, 24(7):  41-51.  DOI: 10.11686/cyxb2014326
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    Phenotypic traits of the natural Gaura parviflora population represent its adaptation to natural environments. Comparison of phenotypic traits among different populations can provide important clues for understanding phenotypic variation and formation processes of G. parviflora. We investigated 16 phenotypic traits in 5 G. parviflora populations located in the coal-gangue pile of Yima and compared variations in phenotypic traits in natural conditions. Our results showed that the average variation coefficient of phenotype traits was 36.30%, which indicated the comparatively abundant phenotypic diversity in G. parviflora. The phenotypic differentiation coefficients (Vst) ranged from 26.85% to 96.98%, the mean value was 73.03%, which indicated that the variance among populations was the main part of the phenotypic variation. Different traits responded differently to environmental changes. Plant height, base stem diameter, number of fruit per plant and specific leaf area were similar among individuals within a populations, but differed from each other significantly among populations. The phenotypic differentiation coefficients (Vst) of them were more than 82%. There were significant differences in the phenotypic traits of internodal space, leaf width, root length, branch number, fruit length and fruit width among populations and among individuals within a population. Number of seeds per fruit and single fruit weight were similar among populations and among individuals within a populations. Plant height, base stem diameter, leaf length, leaf width, stem biomass, leave biomass, root biomass and number of fruit per plant showed a consistent integration pattern, which helped to increase the ecological adaptation of G. parviflora. Cluster analysis showed that different populations from the similar habitat conditions grouped together, further showing that the phenotypic traits of G. parviflora were strongly influenced by the habitat factors. The correlation analysis showed that the majority of phenotypic traits significantly correlated with habitat factors, while the fruit traits (fruit width, single fruit weight and number of seed per fruit) did not, which indicated that reproductive traits were not influenced by the environmental factors.
    Selection of high photosynthetic efficiency Vigna angularis varieties and evaluation of their nutritional value
    ZOU Chang-Ming, WANG Yun-Qing, CAO Wei-Dong, ZHANG Xiao-Hong, LIU Ying, YANG Jie, TANG Shan
    2015, 24(7):  52-59.  DOI: 10.11686/cyxb2014324
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    Field and pot experiments of 7 cultivated Vigna angularis varieties were established in 2011-2013 to evaluate their fertilizer requirement and identify cultivars suitable for Anhui province. The morphology of roots, stems, leaves, flowers, pods, seeds, and also the growth characteristics, yields, and efficiency of dry matter accumulation were surveyed. Photosynthetic efficiency of leaves, weight of root nodules, contents of plant nutrients such as N, P, K, Fe and Zn, were measured at full bloom stage, as were nutritional components crude protein, crude fat, crude fiber and amino acid in the plants. The early-maturing ‘black’ variety of V. angularis (EBVA) matured in the middle of the growing season in Anhui, while the late-maturing ‘black’ cultivar of V. angularis (LBVA) matured in late season. Other varieties matured at an early growth stage. The variety Xingan of the species Adenanthera microsperma (XAM) showed the highest leaf photosynthetic efficiency and dry matter accumulation. LBVA had the highest yield of fresh herbage and dry matter, and highest weight of root nodules, yield of seeds and protein yield LBVA also showed the greatest capacity for accumulation of N, K, Fe and Zn. However, EBVA and XAM had the greatest capacity for P accumulation. Fresh herbage of LBVA was the most palatabile to animals because of its lower crude fiber and higher water content. In conclusion, XAM, EBVA and LBVA were found to be suitable crops for the Anhui area based on photosynthetic efficiency, fresh herbage yield, dry matter yield, nutritional value, capacity for nutrient uptake, and maturity date.
    Utilization of different forms of phosphorus by Sinorhizobium meliloti
    LIU Lu-Sheng, YU Yong-Xiong, GUO Lei, HU Yan, ZHOU Lei, WANG Rong
    2015, 24(7):  60-67.  DOI: 10.11686/cyxb2014322
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    To study the utilization of different forms of phosphorous by acid-tolerant Sinorhizobium meliloti, rhizobia were cultured under three pH conditions; pH of 4.1, 7.0 and 9.0.Solid and liquid culture were used to investigate the growth of rhizobia in different phosphorus media [phosphorus free control (CK), organic phosphorus with yeast extract (CKYE-P), inorganic phosphorus with K2HPO4 (CKP), phytin (TPhy-P), calcium phosphate (TCa-P), aluminum phosphate (TAl-P) and iron phosphate (TFe-P)]. The colony morphology, diameter on solid media, absorbance and available phosphorous and pH in liquid medium were measured. Under all pH conditions, the rhizobia growth in CK was the poorest. Both the colony diameter of CKYE-P and CKP in solid media and the absorbance in liquid media were significantly higher than that in CK, suggesting that phosphorous was the key factor influencing the growth of rhizobia in this experiment. The colony diameter of rhizobiain thephytin treatment (TPhy-P) was largest in all media and pH levels; the aluminum phosphate treatment (TAl-P) and the iron phosphate treatment (TFe-P) produced the smalled colonies. After liquid culturing, the available phosphorus and rhizobia absorbance of TPhy-P as well as TCa-P in neutral and acid medium were much higher than those in alkaline medium. The available phosphorus and rhizobia absorbance of TAl-P and TFe-P were low in all media. The growth of rhizobia led to decreased pH in all media. When compared to the non-inoculated control, available phosphorus enhancement in the inoculated treatment was variable. The results shows that alfalfa rhizobia could effectively utilize phosphorous in the form of phytin and calcium orthophosphate, but that the availability of phosphorous under alkaline conditions declined dramatically. The phosphorous inaluminum phosphate and ironic phosphate was unavailable to alfalfa rhizobia. Media pH had a big influence on the availability of phytin and calcium orthophosphate but had little effect on the availability of aluminum phosphate and iron phosphate. Rhizobia growth could result in decreased media pH and increased available phosphate, but this was affected by phosphorus form.
    Response of the antioxidant system to water stress in different wheat varieties
    SHI Zhen-Zhen, LI Sheng, MA Shao-Ying, WANG Ya-Mei, SU Li-Wei, TANG Bin, ZHAO Sheng-Qin, SU Li-Rong
    2015, 24(7):  68-78.  DOI: 10.11686/cyxb2014342
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    In this study, the concentration or activity changes of some drought indicators, including proline (Pro), soluble sugar, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and superoxidase (POD) were measured with two wheat varieties “Yongliang 4” and “Xihan 2” under water deficit stress. The contents of Pro and MDA increased and the soluble sugar content rose initially and then fell with increased degree and duration of water stress. The MDA content in “Yongliang 4” increased more than that in “Xihan 2”. The activities of SOD, CAT and POD increased under mild water stress, and activities of these enzymes in “Xihan 2” increased more than those in “Yongliang 4”. However, the activities of the three enzymes rose initially and then decreased under moderate water stress. All the indicators showed differing recovery patterns after rehydration, and compared with “Yongliang 4”, the overall drought resistance and recovery response of “Xihan 2” was better. The degree of change of the above stress indictors was ranked: leaf>sheath>stem, showing that the most sensitive organ to water stress was the leaf, and the least sensitive was the stem.
    Effects of drought stress and rewatering on the growth and physiological characteristics of Shanxi wild Poa pratensis seedlings
    ZHU Hui-Sen, WANG Bao-Ping, DONG Xiao-Yan, DONG Kuan-Hu, LI Cun-Fu
    2015, 24(7):  79-88.  DOI: 10.11686/cyxb2015098
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    Research has been undertaken on the effects of drought and rewatering on the growth and physiological characteristics of 15 species wild Poa pratensis seedlings in Shanxi. Using pot tests, at the four-leaf stage the P. pratensis species were water-controlled for 21 days and then re-watered. Seedling survival rates, relative growth rates (RGR), relative water content (RWC), proline (Pro), and the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) content were measured on the 21st day after water-controlling and the 7th day after rewatering. All indexes were evaluated with the subordination function method to obtain the membership value of each population. Results showed that seedling survival rates, RGR, RWC and SOD declined during the 21-day drought stress. However, Pro and MDA contents increased. After rewatering, all the indexes rehabilitated to some degree but did not recover to the same level as the control groups. Data analysis showed the tested P. pratensis species could be grouped into three kinds. Class Ⅱ were medium drought-resistant populations, including QinshuiⅡ, HunyuanⅠ, QinshuiⅠ, Wutai, HunyuanⅡ, Yingxian, Guangling and Lingqiu. Class Ⅲ showed weaker drought resistance, including the Yuanqu, Ningwu, Huozhou, Xinzhou, Wuzhai and Lingshi populations. The Tunliu population was categorized as class Ⅳ, which had no drought resistance. The membership value of the QinshuiⅡpopulation was the highest, and could be used as the key selection object in the future.
    Effects of calcium (CaCl2), GA3 and complex liquid on the physiological characteristics of alfalfa seedlings under drought stress
    GUO Yu-Pin, REN Yong-Xia, LIU Gui-He, CAO Chun-Mei, YAN Li-Jun
    2015, 24(7):  89-96.  DOI: 10.11686/cyxb2014502
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    This study aimed to investigate the effects of calcium (CaCl2) and GA3 on alfalfa seedlings’ physiological characteristics under drought stress and to discover appropriate concentrations for alleviating plant damage. Alfalfa seedlings were cultured in sand. The tested concentrations for CaCl2 were 0, 5, 10 and 20 mmol/L; for GA3 they were 0, 50, 100 and 150 mg/L, also tested was a complex liquid of 10 mmol/L CaCl2 with 100 mg/L GA3 (CaCl2:GA3). These four treatments (T1∶1, T1∶2, T2∶1 and Z1∶1 respectively) were investigated under conditions of PEG-6000 drought stress. Results indicated that chlorophyll relative content decreased under stress, while relative electrolytic leakage and MDA, Pro, SOD and POD contents increased significantly. Appropriate concentrations of CaCl2, GA3 and complex liquid could alleviate the degradation of seedling chlorophyll and reduce the relative conductivity and accumulation of MDA that protect film stability and reduce the content of osmotic regulation substances. They can also maintain higher SOD and POD activity and significantly improve alfalfa seedling resistance to drought stress. Using the method of subordinate function, it was found that 10 mmol/L CaCl2, 100 mg/L GA3 and CaCl2+GA3 T1∶1 performed better than 10 mmol/L CaCl2 and 100 mg/L GA3. The study concluded that the relative drought relief effect of the treatments from strong to weak was T1∶1>10 mmol/L CaCl2>T2∶1>CK1>T1∶2>100 mg/L GA3>Z1∶1>CK2.
    Effects of salicylic acid on seed germination and seedling physiological characteristics of Zinnia elegans under salt stress
    HUANG Yu-Mei, ZHANG Yang-Xue, LIU Qing-Lin, LIU Pan, HUANG Sheng-Lan
    2015, 24(7):  97-105.  DOI: 10.11686/cyxb2014516
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    Zinnia elegans seed (cultivar “Fangfei No.1”) was treated with 100 mmol/L NaCl. The effects of different concentration of exogenous salicylic acid (SA: 0, 0.5, 1.0, 1.5, and 2.0 mmol/L) on salt stress-injuries were investigated during seed germination and seedling growth. The results indicated that SA can effectively alleviate the injuries of salt stress on seed germination and seedling growth of Z. elegans. Salicylic acid increased germination vigour, germination percentage, germination index, and subsequently increased plant height, stem diameter, root∶shoot ratio, chlorophyll content, proline, soluble sugar and soluble protein content and superoxide dismutase (SOD) and peroxide (POD) activity under salt stress. However, malondialdehyde (MDA) content (indicator of oxidative stress) was decreased by salicylic acid. Salicylic acid concentration of 1.0 mmol/L produced the best effects whereas no mitigation effects were observed on seed germination or seedling growth under salt stress at 2.0 mmol/L salicylic acid. It is suggested that salicylic acid concentrations of 0.5-1.5 mmol/L could be used to alleviate salt stress-induced injuries.
    The effects of dry storage at room temperature and stratification at natural alpine meadow on seed germination of Anemone rivularis
    YU Xiao-Jun, WANG Fang, ZHANG Jing, XU Chang-Lin, XIAO Hong, JING Yuan-Yuan, ZHANG Jian-Wen, YANG Hai-Lei
    2015, 24(7):  106-115.  DOI: 10.11686/cyxb2014430
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    In order to clarify conditions needed for the seed dormancy release of Anemone rivularis, and provide reference information for grassland management and A. rivularis cultivation, the effects on A. rivularis seed germination characteristics of dry storage at room temperature and stratification in natural conditions in an alpine meadow were studied. Storage time, peeling and illumination affected the time required for germination, germination percentage and germination index of A. rivularis seed. With increased storage time, time required for germination of peeled A. rivularis seeds decreased and the germination percentage and germination index increased to different degrees. With illumination, the number of days required for germination of both peeled and unpeeled A. rivularis seeds stored for 40 to 180 days was significantly lower (P<0.05) than that of the same seeds without storage. There were no significant differences in the germination of peeled A. rivularis between seeds stored for 40, 80, 150 or 180 days although germination was always significantly higher (P<0.05) than that of same seeds without storage. The germination of unpeeled A. rivularis seeds stored at room temperature for 180 days was significantly higher (P<0.05) than that of same seeds stored for 80 and 150 days, which in turn was significantly higher (P<0.05) than that of same seeds stored for 40 days. All of the germination percentages of unpeeled A. rivularis seeds with storage were significantly higher (P<0.05) than those of same seeds without storage. Both illumination and stratification in a natural alpine meadow during the cold season reduced the days required for germination of A. rivularis seeds and improved germination percentage and germination index. The time required for initial germination of A. rivularis seeds with stratification and trampling and subject to alternating temperatures of 30/20℃or 20/10℃ was significantly shorter (P<0.05) than that of seeds subjected to stratification without trampling under the same germination conditions. The germination of A. rivularis seeds with treatments comprising stratification and trampling at alternating temperatures of 25/15℃ and 20/10℃was 38.0% and 35.3%, respectively, which was significantly higher (P<0.05) than that of treatments comprising stratification without trampling under the same germination conditions (22.7% and 14.0%, respectively). However, unpeeled A. rivularis seeds stored dry at room temperature in the same period (November to April) did not germinate. Un-germinated A. rivularis seeds had a 40.2% germination rate after stratification in Tianzhu alpine meadow from October to August in the following year. Un-germinated seeds could also germinate under alternating temperature regimes ranging from 30/20℃ to 20/5℃ with the highest observed germination percentage being 15.3%at 30/20℃.This indicates that the majority of seeds still remained dormant after initial treatment.
    Effect of temperature and additives on fermentation and α-tocopherol and β-carotene content of Pennisetum purpureum silage
    LIU Qin-Hua, LI Xiang-Yu, LI Jun-Feng, BAI Xi, ZHANG Jian-Guo, SHAO Tao, WU Lin, ZHAO Xin-Guo, TIAN Jia-Lu
    2015, 24(7):  116-122.  DOI: 10.11686/cyxb2014338
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    The effects of temperature (45, 30, 15℃) and additives (no additives: CK; cellulase: E; Lactobacillus plantarum: LP), on the fermentation and α-tocopherol and β-carotene content of Pennisetum purpureum silages was determined. The results indicated that the P. purpureum silage produced good fermentation at all temperatures; ensiling at 30℃ produced less butyric acid however. Additives (LP and E) produced significantly higher lactic acid content, lactic acid to acetic acidratio and lower pH (P<0.05) than silages without additive (CK), indicating additives can improve fermentation quality of P. purpureum silage. The study revealed that P. purpureum silage fermented at 30℃ had significantly higher β-carotene, but lower α-tocopherol content than silages fermented at 45 and 15℃ (P<0.05). Silage supplemented with LP and E had lower α-tocopherol and β-carotene contents (P<0.05). E treated silage had higher α-tocopherol content at 30℃ (P<0.05) and higher β-carotene content at 30℃ and 15℃ (P<0.05) than LP treated silage.
    The effects of Meloidogyne incognita infection on osmolyte and hydroxyproline levels in tomato rootstock seedlings with different resistance
    JIA Shuang-Shuang, XU Kun
    2015, 24(7):  123-130.  DOI: 10.11686/cyxb2014333
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    In order to clarify the physiological resistance mechanism of tomato rootstock to Meloidogyne incognita, both a highly susceptible rootstock variety Ls-89 and a highly resistant rootstock variety Banzhen No.2 were planted in pots and inoculated with second instar larvae to study the effects of M. incognita infection on osmolyte levels and cell wall hydroxyproline contents of these differentially resistant tomato rootstock varieties. Without M. incognita inoculation, there was no significant difference (P>0.05) between seedlings of the two varieties in concentrations of osmoregulation substances, including soluble sugar, soluble protein, proline and cell wall hydroxyproline. After inoculation, the concentrations of osmoregulation substances and cell wall hydroxyproline contents were increased in roots and leaves in seedlings of the two varieties, with the responses in Banzhen No.2 being significantly higher (P<0.05) than those of Ls-89. These results indicate that changes in soluble sugar, soluble protein, proline and cell wall hydroxyproline concentrations are closely associated with the resistance of tomato rootstock seedlings to M. incognita, and could be used as general indices of plant resistance elevation.
    Proteomic methods for removing high-abundance proteins in alfalfa leaf
    CHEN Jing, HAN Gui-Qing, SHAN Chen, ZHAN Hai-Ling, LI Ji-Kai, LIU Hui-Ying, ZHANG Yue-Xue
    2015, 24(7):  131-138.  DOI: 10.11686/cyxb2015044
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    The higher content of high-abundance proteins (Ribulose-l, 5-bisphosphate carboxylase/oxygenase; RuBisCo) interferes with the dynamic resolution of proteins in two-dimensional electrophoresis (2-DE), affecting the detection and identification of functional proteins in proteomics. To explore suitable methods for removing high-abundance protein, Mg/NP-40 and different concentrations of polyethylene glycol (PEG) were used for protein pre-fractionation and the treatments’ influence on the separation of proteins in alfalfa leaf was compared. The results indicated that (335±17), (417±3), (445±7) and (459±11) spots were detected in treatments of 0, 15%, 17.5% and 20% PEG respectively. There were significant differences between the 0-17.5% treatments but no significant differences were found between the 17.5% and 20% treatments. More differential protein spots were detected in the 17.5% treatment, which thus proved the most effective way of removing RuBisCo proteins. In order to inspect the applicability of this result, a proteomic study of alfalfa in response to low temperatures was under taken. Compared with total proteins extracted by TCA/acetone, eight new protein spots were identified after PEG treatment to remove high-abundance proteins. The research thus indicates that pre-fractionation with Mg/NP-40 and17.5% PEG is suitable for proteomic studies of alfalfa.
    A study of anatomical structure and apoplastic barrier characteristics of Hydrocotyle sibthorpioides
    YANG Chao-Dong, LI Shou-Feng, YAO Lan, AI Xun-Ru, CAI Xiao-Dong, ZHANG Xia
    2015, 24(7):  139-145.  DOI: 10.11686/cyxb2014246
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    The anatomy and histochemistry of Hydrocotyle sibthorpioides were investigated under light and fluorescence microscopy in this research. The findings were: 1) The adventitious root of H. sibthorpioides consisted of proto-xylem and proto-phloem, superceded by diarch primary vasculature of metaxymlem and metaphloem, surrounded by endodermis, then cortex, exodermis and epidermis. 2) Stolon, pedicel and leaf were primary structures with epidermis, collenchyma, cortex, endodermis, vascular bundle and pith, except that vasculature of the stolon and pedicel had secondary structures, and stolon had induced aerenchyma. 3) The apoplastic barriers in adventitious roots of H. sibthorpioides included the inner layers of endodermis with Casparian bands and suberin, the outer layers of exodermis with Casparian bands and suberin and the epidermis with suberin. The stolons, pedicels and petioles had similar apoplastic barriers, including the inner layer of endodermis with Casparian bands and suberin, and the outer layer of the cuticle, but the endodermis had no suberin in pedicels and petioles; leaf blades had only a cuticular apoplastic barrier. 4) The anatomical structure and apoplastic barrier characters are important structural determinants of adaptation of H. sibthorpioides to variably-moist environments. The implications of this research for selection of plant species to restore degraded wetlands are discussed.
    Development and functional verification of cSSR markers for recessive genetic male sterility in oats
    ZHANG Li-Jun, LIU Long-Long, CHANG Zhi-Jian, GUO Bin, CUI Lin
    2015, 24(7):  146-154.  DOI: 10.11686/cyxb2014232
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    The objective of this study was to develop new cSSR markers from an oat (Anena sativa) recessive genes male sterile transcriptome database. EST sequences obtained from a RNA-Seq of oat male sterile near-isogenic line CAMS9 were used to search for SSRs using software. cSSR primers were developed and functional verification for those related to male sterility was determined with a F2 population of hybridized CAMS9 and Pinyan 2.6409 EST sequences obtained from the transcriptome sequencing had SSR loci and 736 of these sequences had 2 or more loci. Most of the sequence motifs were dinucleotides (56.66%) and trinucleotides (24.30%). For trinucleotides, CCG/CGG (26.68%), AGG/CTT (21.29%), AGC/CTG (18.48%), AAG/CTT (8.18%) and ACC/GGT (8.11%) were the most frequent repeats. For dinucleotides, AG/CT (60.63%) and AC/GT (26.24%) were the most frequent. Based on these cSSRs, 13344 pairs of cSSR primers were designed. 45 pairs of the primers were synthesized based on male sterile-related cSSRs, and 32 (71.1%) of the pairs led to amplified products. Using the F2 population, the polymorphism of 11 pairs between parents and the differences of 7 pairs between fertile and sterile trait pools were detected. In total, 13344 pairs of cSSR primers have been developed from the RNA-Seq database. 32 pairs were related to fertility and 7 can be used for molecular detection of oat male sterility. These new cSSR markers are beneficial for the further use of oat germplasm and for the study of male sterility.
    An evaluation of the resistance of alfalfa cultivars to Stemphylium and Phoma leaf spot diseases
    WANG Yu, LIU Yi, ZHOU Bin-Bin, YUAN Qing-Hua, ZHANG Li, PAN Long-Qi
    2015, 24(7):  155-162.  DOI: 10.11686/cyxb2014337
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    Forty alfalfa (Medicago sativa) cultivars were collected from China and abroad and inoculated with suspensions of different concentrations of spores from Stemphylium leaf spot and Phoma leaf spot diseases to evaluate the resistance of alfalfa to these two leaf spot diseases and identify the optimal inoculation concentration. The disease indices of 10 cultivars inoculated with these two fungi gradually increased with concentration increase of the spore suspensions. The appropriate inoculation concentrations for comparing cultivar resistance to Stemphylium and Phoma were 1×105 to 1×106 spores per milliliter and 1×106 spores per milliliter, respectively. With a concentration of 1×106 spores per milliliter in the inoculate, the disease indices of Stemphylium leaf spot in forty cultivars ranged from 3.33 to 35.83, while the disease indices of Phoma leaf spot ranged from 13.07 to 52.27. There were significant differences between the 40 cultivars (P<0.05). Fourteen cultivars were found to be resistant to Stemphylium leaf spot, including one highly-resistant cultivar-Gongnong No.2 and thirteen moderately-resistant cultivars. Only one cultivar, Rambler, from America was found to have moderate resistance to Phoma leaf spot.
    Production and international trade analysis of animal products in China
    ZHANG Zhong-Li, YU Ying-Wen, FU Qiang-Guo, SUN Yi, HOU Fu-Jiang
    2015, 24(7):  163-171.  DOI: 10.11686/cyxb2012100
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    A systematic analysis on the production and international trade of the major animal products in China and the world and of the non-traditional animal products in China showed an increasing trend in the past 20 years as shown by the network database information of the Food and Agriculture Organization of the United Nations (UNFAO) from 1990 to 2009. During this time, world meat production and that from China increased from 17.99 ten million tons and 30 to 78 million tons and from 17.99 to 28.39 ten million tons respectively with the proportion from China increasing from 16.9% to 27.5%. World egg production increased by 3.52 ten million tons to 6.28 ten million tons, with the proportion from China increasing from 18.61% to 37.61%; In the past 20 years, China’s milk production increased by 8 times, the wool production of the world fell from 3.35 million tons to 2.04 million tons, and the wool production of China increased from 23.94 ten thousand tons to 36.40 ten thousand tons. The net trade deficit of beef increased from 478 tons to 1694 tons. The trade deficit quantity and the trade deficit value of sheep and goat meat in 2009 were 76.5 thousand tons worth 156.6 million dollars, compared to 1990 these are increases of 745.6% and 1102% respectively. In addition in the years 2000 and 2006-2008, the quantity and value of Chinese pork import and export were in surplus; Chinese poultry imports were far more than the exports; the trade value from 1990 to 2006 was in surplus, but from 2007 to 2009 it was in deficit. The egg export quantity from 2000 to 2009 continued to increase to 134.7 thousand tons, and the egg import quantity in 2009 was 268 tons; The milk trade of China from 1990 to 2009 was in surplus, its import quantity and value in 2009 increased to 14.6 thousand tons and 11.8 million dollars, increasing by 80.9% and 64.05% respectively compared with 2008. The wool trade of China from 1990 to 2009 was in deficit, with the deficit quantity and value increasing from 35.2 thousand tons and 127.4 million dollars to 269.2 thousand tons and to 136.8 million dollars. Animal production was closely related to that of forage. Due to animal diseases, drug residues, and animal welfare issues, the animal products trade of China is not dominant in the world. We need to improve production standards through feed production, aquaculture production, meat processing, circulation and other aspects, in order to promote animal products economic development.
    Review of bud banks in grassland ecosystems
    ZHAO Ling-Ping, WANG Zhan-Bin, CHENG Ji-Min
    2015, 24(7):  172-179.  DOI: 10.11686/cyxb2014332
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    Basal budsare important for vegetative reproduction, influencing seasonal growth, population regeneration and community dynamics of aboveground vegetation in grassland ecosystems, especially in clonally plant-dominant perennial grasslands. Bud bank characterization, including influencing factors, is an important aspect of plant ecology which will benefit the prediction of vegetation succession, and inform vegetation management. The review summarizes the size, composition, spatial and temporal pattern of grassland bud banks and its influencing factors, providing useful direction for future research.
    Review of advances in the study of plant ABCG transporters
    ZHANG Jing, CHEN Meng-Ci, MA Qing, WEI Li, WANG Suo-Min
    2015, 24(7):  180-188.  DOI: 10.11686/cyxb2014415
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    The ATP-binding cassette (ABC) transporters are members of a protein superfamily that constitutes one of the largest protein families known in plants. The ABC subfamily G (ABCG) consists of a single ABC cassette in the amino terminal. ABCG includes both the half-size molecular transporter white-brown complex (WBC) and the full-size molecular transporter pleiotropic drug resistance (PDR). ABCG is made up of a wide variety of substances (including antibiotics, phytohormones, lignin monolignols, lipids and secondary metabolites) that are involved in many kinds of metabolic processes during the plant life cycle. This paper reviews recent advances in studies of the molecular structure and function of ABCG transporters. Research hotspots and future directions are also considered.
    Evaluation of food security and new developmental routing selection
    TANG Yu-Tong, LIN Hui-Long
    2015, 24(7):  189-196.  DOI: 10.11686/cyxb2013243
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    The traditional Chinese food security concept equates grain to food and our agricultural policy lays undue stress on the importance of grain, which accelerates the formation of the “grain-pig agriculture”. The food production structure and safety evaluation methods can reflect the drawbacks of traditional agriculture: on the one hand we pay too much attention to grain and pork production while on the other hand, the evaluation of food security is confined to the food supply capacity. Foreign food security evaluation systems focus on micro-level and nutrition structure, but domestic evaluation systems prefer macro-level and food supply capacity. Traditional agriculture with a single production structure no longer meets the people’s need for increased animal food. We do not have enough grain to feed large numbers of grain-eating livestock if we still continue to give priority to the development of confined animal husbandry. A good solution for this problem is to develop plant-eating livestock to relieve the pressure on grain-eating livestock. Grassland agricultural systems can ensure food security not only in quantity, but also to satisfy the demand of increasing animal food consumption. Thus, it is the best choice of development for efficiently protecting our country’s food security in the long term.
    Plant diversity patterns along altitudinal gradients in alpine meadows in the Three River Headwater Region, China
    LU Hui, CONG Jing, LIU Xiao, WANG Xiu-Lei, TANG Jun, LI Di-Qiang, ZHANG Yu-Guang
    2015, 24(7):  197-204.  DOI: 10.11686/cyxb2014347
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    The Three River Headwater region of Qinghai province, also known as the Qinghai-Tibetan Plateau is one of the regions in China likely to be most significantly affected by climate change. Hence, understanding the relationship between plant community distribution patterns and environment factors could afford scientific evidence for predicting the effect of climate change on plant diversity. In this research, six sites at a range of elevations and including 78 alpine meadow plots in the Three River Headwater region were surveyed to study the relationship between plant community structure and the environment. We aimed to explore the potential plant responses to climate change using a strategy of space-for-time substitution. At the six sites a total of 74 plant species belonging to 21 families and 51 genera were identified. Cyperaceous plants such as Kobresia schoenoides, Kobresia pygmaea and Kobresia humilis were the dominant species in the middle-high-elevation area, while plants of the Poaceae such as Stipa capillata, Poa annua and Elymus nutans covered the most of low elevation area. Species diversity index and richness index of the alpine meadow sites showed a unimodal pattern, and species evenness showed no significant differences along with the altitudinal gradient across the six sites. Detrended correspondence analysis indicated that species composition and diversity of plant communities changed continuously with decreasing elevation from alpine meadow to alpine steppe meadow. In addition, canonical correspondence analysis showed that soil pH was the main factor affect species diversity patterns in alpine meadow. Meanwhile, soil moisture, temperature, organic carbon content, available nitrogen content and total potassium content were key factors affect the distribution patterns of plant communities. On the basis of these observations, we predict that climate change is very likely to change plant community structure in the Three River Headwater Region.

    Plastic covered sheds for drying alfalfa
    YIN Qiang, JIA Yu-Shan, YAN Zhi-Jian, WANG Yu-Qing, CHANG Chun, WANG Zhi-Jun, WANG Hui
    2015, 24(7):  205-211.  DOI: 10.11686/cyxb2014335
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    Alfalfa (cv. Suntoryin) was dried in clear plastic covered sheds to investigate the benefits for alfalfa hay production in the Huanghuaihai region. Air temperatures in the plastic covered shed were 27-46℃ higher than the outside temperature (5-13℃); there lative humidity under plastic was 16%-52% lower than that outside indicating that plastic covered sheds could increase alfalfa drying rate, helping to maintain forage value while eliminating the risk of high night humidity and rain. The moisture content of alfalfa dried under plastic was reduced to 15% after 32 hours, 48 hours less than the control. The crude protein and total digestible nutrient contents were 18.64% and 66.72%, 20.73% and 11.00% higher than the control, respectively; neutral detergent fiber and acid detergent fiber contents were 41.89% and 29.65%, 12.60% and 17.87% lower than the control, respectively. Alfalfa dried under plastic dried more quickly and was of higher quality suggesting that the use of plastic covered drying sheds would be advantageous for farmers with relatively small areas of alfalfa who live in high rainfall areas.