Response of the antioxidant system to water stress in different wheat varieties

doi:10.11686/cyxb2014342

Abstract

Abstract: In this study, the concentration or activity changes of some drought indicators, including proline (Pro), soluble sugar, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and superoxidase (POD) were measured with two wheat varieties “Yongliang 4” and “Xihan 2” under water deficit stress. The contents of Pro and MDA increased and the soluble sugar content rose initially and then fell with increased degree and duration of water stress. The MDA content in “Yongliang 4” increased more than that in “Xihan 2”. The activities of SOD, CAT and POD increased under mild water stress, and activities of these enzymes in “Xihan 2” increased more than those in “Yongliang 4”. However, the activities of the three enzymes rose initially and then decreased under moderate water stress. All the indicators showed differing recovery patterns after rehydration, and compared with “Yongliang 4”, the overall drought resistance and recovery response of “Xihan 2” was better. The degree of change of the above stress indictors was ranked: leaf>sheath>stem, showing that the most sensitive organ to water stress was the leaf, and the least sensitive was the stem.

SHI Zhen-Zhen, LI Sheng, MA Shao-Ying, WANG Ya-Mei, SU Li-Wei, TANG Bin, ZHAO Sheng-Qin, SU Li-Rong. Response of the antioxidant system to water stress in different wheat varieties[J]. Acta Prataculturae Sinica, 2015, 24(7): 68-78.

References

[1] Dai J J, Wang Y T. Agricultural Mitigation Guide[M]. Beijing: Chinese Agricultural Press, 1994.
[2] Li X Q, Jin S H. Effects of drought stress on gas exchange and chlorophyll fluorescence characteristics in Eremochloa ophiuroides . Chinese Agricultural Science Bulletin, 2010, 26(8): 170-174.
[3] Ji Y, Zhang X Q, Peng Y, et al . Effects of drought stress on lipid peroxidation, osmotic adjustment and activities of protective enzymes in the roots and leaves of orchardgrass. Acta Prataculturae Sinica, 2014, 23(3): 144-151.
[4] Hall A E. Physiological Ecology of Crops in Relation to Light, Water and Temperature[M]. New York: McGrawHill Publishing Company, 1990: 191-233.
[5] Zhang C J, Ji T H, Yang Z G, et al . Study on resistance drought identify method and evaluation index of wheat Ⅰidentify method and evaluation index. Chinese Agricultural Science Bulletin, 2007, 23(9): 226-230.
[6] Li D Q, Guo Q F, Zhang Y Q, et al . Studies on the physiological characteristics of drought resistance in winter wheat. Acta Agronomica Sinica, 1993, 19(2): 125-132.
[7] Jiang D, Yu Z W, Li Y Q, et al . Changes of soluble sugar contents in leaf, stem and grain in winter wheat and its relationship with grain starch accumulation. Journal of Triticeae Crops, 2001, 21(3): 38-41.
[8] Zhang J, Liu H, Li X P, et al . Effect of drought on leaf physiological parameters and yield of wheat at booting stage. Agricultural Research in the Arid Areas, 2014, 32(3): 1-8.
[9] Huang C B, Zeng F J, Lei J Q. Comparison of drought resistance among three Calligonum in the Southern Fringe of the Taklamakan Desert. Acta Prataculturae Sinica, 2014, 23(3): 136-143.
[10] DaCosta M, Huang B R. Changes in antioxidant enzyme activities and lipid peroxidation for Bentgrass species in response to drought stress. Journal of the American Society for Horticultural Science, 2007, 132: 319-326.
[11] Sairam R K, Vasanthan B, Arora A. Calcium regulates gladiolus flower senescence by influencing antioxidative enzymes activity. Acta Physiologiae Plantarum, 2011, 33(5): 1897-1904.
[12] Zhang X H, Huang C L, Shen Y Y, et al . Research progress in plant drought resistant gene engineering. Biotechnology Information, 2001, (4): 21-25.
[13] Wang J L, Zhang X Z, Su Z J, et al . Physiological responses and mechanisms of wheat to drought resistance. Acta Tritical Crops, 1994, (5): 44-46.
[14] Fei M H. Effect of water stress on seeding growth and antioxidant ability of wheat. Journal of Jilin Normal University (Natural Science Edition), 2011, (1): 43-44.
[15] Gao J F. Plant Physiology Experimental Guidance[M]. Beijing: Higher Education Press, 2006.
[16] Chandra B R, Safiullah P M, Blum A, et al . Comparison of measurement methods of osmotic adjustment in rice cultivars. Crop Science, 1999, 39: 150-158.
[17] Hsiao T C, O’Toole J C, Yambao E B, et al . Influence of osmotic adjustment on leaf rolling and tissue death in rice. Plant Physiology, 1984, 75(2): 338-341.
[18] Zhang M S, Peng Z H, Xie B, et al . Relationship between water loss rate of cutting leaves and osmotic regulators under water stress and drought resistance in sweet potato. Scientia Agricultura Sinica, 2004, 37(1): 152-156.
[19] Yang S Y, Yan P, Mei X Y. The impact on soluble sugar and proline contents under different water stress. Chinese Agricultural Science Bulletin, 2007, (12): 229-233.
[20] Zhao W C, Li H, Zhao H J, et al . Effects of exogenous polyamine osmotica regulation in wheat seedling leaves under drought stress. Chinese Agricultural Science Bulletin, 2009, 25(9): 148-151.
[21] Xiong J Y, Zhang Z M, Bai T, et al . Comprehensive evaluation of drought resistance of wheat mutant lines derived from ultra-high-pressure mutagenesis. Acta Agriculturae Boreali-Occidentalis Sinica, 2014, (6): 81-87.
[22] Wang Y, Di X Y, Ma J P, et al . Drought resistance of seedlings of eight provenances of Chinese pine. Bulletin of Soil and Water Conservation, 2009, 29(4): 46-50.
[23] Wang B S, Zhao S Q. Effect of drought on lipid peroxidation and defensive enzymes in the wheat seedlings. Journal of Shandong Normal University (Natural Science), 1987, (1): 29-30.
[24] Ma Z P, Li M F, Yang D L, et al . Relationship between grain filling and accumulation and remobilization of water soluble carbohydrates in leaf and stem of winter wheat during the grain filling in different water conditions. Acta Prataculturae Sinica, 2014, 23(4): 68-78.
[25] Song X Y, Wu S, Zhang H S, et al . Effect of soil water stress on physiological characteristics in different winter wheat cultivars. Acta Agriculturae Boreali-Sinica, 2014, 29(2): 174-180.
[26] Wang W L, Lin Z P, Zhang X Y, et al . On the effect of soil drought during the period from the end of flowering to ripening on the grain filling and matter translocation in the wheat plant. Acta Phytophysiologia Sinica, 1982, 8(1): 67-80.
[27] Zhang W, Gao W, Cao Z, et al . Immunolocalization and quantitation of ABA and IAA in the organs of wheat ( Triticum aestivum L.) under drought stress. Scientia Agricultura Sinica, 2014, 47(15): 2940-2948.
[28] Wei A L, Wang Z M, Chen B, et al . Effect of soil drought on electron transport rate and photophosphorylation level of different green organs in wheat. Acta Agronomica Sinica, 2004, 30(5): 487-490.
[1] 代久江, 王咏涛. 农业减灾指南[M]. 北京: 中国农业出版社, 1994.
[2] 李雪芹, 金松恒. 干旱胁迫对假俭草气体交换和叶绿素荧光特性的影响. 中国农学通报, 2010, 26(8): 170-174.
[3] 季杨, 张新全, 彭燕, 等. 干旱胁迫对鸭茅根、叶保护酶活性、渗透物质含量及膜质过氧化作用的影响. 草业学报, 2014, 23(3): 144-151.
[5] 张灿军, 冀天会, 杨子光, 等. 小麦抗旱性鉴定方法及评价指标研究Ⅰ. 鉴定方法及评价指标. 中国农学通报, 2007, 23(9): 116-230.
[6] 李德全, 郭清福, 张以勤, 等. 冬小麦抗旱生理特性的研究. 作物学报, 1993, 19(2): 125-132.
[7] 姜东, 于振文, 李永庆, 等. 冬小麦叶茎粒可溶性糖含量变化及其与籽粒淀粉积累的关系. 麦类作物学报, 2001, 21(3): 38-41.
[8] 张军, 刘红, 李晓萍, 等. 干旱对小麦孕穗期叶片生理特性及产量的影响. 干旱地区农业研究, 2014, 3(3): 1-8.
[9] 黄彩变, 曾凡江, 雷加强. 塔克拉玛干沙漠南缘3个沙拐枣种的抗旱特性比较. 草业学报, 2014, 23(3): 136-143.
[12] 张秀海, 黄丛林, 沈元月, 等. 植物抗旱基因工程研究进展. 生物技术通报, 2001, (4): 21-25.
[13] 王金铃, 张宪政, 苏正椒, 等. 小麦对干旱的生理反应及抗性生理. 国外农学-麦类作物, 1994, (5): 44-46.
[14] 费明慧. 水分胁迫对小麦幼苗生长及抗氧化能力影响. 吉林师范大学学报(自然科学版), 2011, (1): 43-44.
[15] 高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[18] 张明生, 彭忠华, 谢波, 等. 甘薯离体叶片失水速率及渗透调节物质与品种抗旱性的关系. 中国农业科学, 2004, 37(1): 152-156.
[19] 杨书运, 严平, 梅雪英. 水分胁迫对冬小麦抗性物质可溶性糖与脯氨酸的影响. 中国农学通报, 2007, 23(12): 229-233.
[20] 赵文才, 李慧, 赵会杰, 等. 外源腐胺对干旱胁迫下小麦叶片渗透调节的影响. 中国农学通报, 2009, 25(9): 148-151.
[21] 熊建云, 张正茂, 白婷, 等. 超高压诱变小麦突变株系的抗旱综合评价. 西北农业学报, 2014, (6): 81-87.
[22] 王琰, 狄晓艳, 马建平, 等. 8个油松种源抗旱性的比较研究. 水土保持通报, 2009, 29(4): 47-50.
[23] 王宝山, 赵思齐. 干旱对小麦幼苗膜脂过氧化及保护酶的影响. 山东师范大学学报(自然科学版), 1987, (1): 29-30.
[24] 马召朋, 栗孟飞, 杨德龙, 等. 不同水分条件下冬小麦灌浆期茎叶可溶性碳水化合物积累转运与籽粒灌浆的关系. 草业学报, 2014, 23(4): 68-78.
[25] 宋新颖, 邬爽, 张洪生, 等. 土壤水分胁迫对不同品种冬小麦生理特性的影响. 华北农学报, 2014, 29(2): 174-180.
[26] 王万里, 林芝萍, 张秀英, 等. 灌浆-成熟期间土壤干旱对小麦籽粒充实和物质运转的影响. 植物生理学报, 1982, (1): 67-81.
[27] 张炜, 高巍, 曹振, 等. 干旱胁迫下小麦( Triticum aestivum L.)幼苗中ABA和IAA的免疫定位及定量分析. 中国农业科学, 2014, (15): 40-48.
[28] 魏爱丽, 王志敏, 陈斌, 等. 土壤干旱对小麦绿色器官光合电子传递和光合磷酸化活力的影响. 作物学报, 2004, 30(5): 487-490.