Karyotype analysis and mitotic observation of Elymus sibiricus

doi:10.11686/cyxb2014371

Abstract

Abstract: A study has been undertaken to determine the karyotype of Elymus sibiricus. The results showed that the chromosome number of E. sibiricus was 28, with a pair of satellites on the short arms of the 13^th chromosomes. Its karyotype formula is 2n=4x=24m+4sm (2SAT). The percentage of chromosomes with arm ratios over 2 was 7.14%, and the ratio of the longest to the shortest was 2.04. Its karyotype asymmetry is 2B. There were five phases (Interphase, Prophase, Prometaphase, Metaphase and Anaphase) in the process of mitosis. At interphase the nuclei were dyed homogeneously. During prophase the shape of the slender chromosomes was visible. The individual chromosome could be identified clearly in the prometaphase. At metaphase the chromosomes highly concentrated and the chromatids and centromeres were distinctly visible. During anaphase the chromatids separated and moved to the opposite poles.

ZHENG Hui-Min, MAO Pei-Sheng, HUANG Ying. Karyotype analysis and mitotic observation of Elymus sibiricus[J]. Acta Prataculturae Sinica, 2015, 24(8): 225-229.

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