Identification of cyst nematode in alpine meadow steppe, Gansu

doi:10.11686/cyxb2014372

Abstract

Abstract: A survey of grass cyst nematodes in Tianzhu alpine meadow steppe, Gansu Province was conducted in August, 2013. Cyst nematodes were isolated from the roots and root rhizosphere of Kobresia sp. and Kobresia humilis. The nematodes were initially identified as Heterodera avenae based on cyst characteristics, particularly the cyst vulva cones. The cysts were light brown, large lenmon-shaped and contained vulva cones with bifenestrate and obvious bullae and without a vulva under-bridge. The 1045 bp sequence fragment of ITS region was amplified using PCR with general primers AB28 and TW81. The similarities between these groups and H. avena were 99%-100%. Molecular analysis confirmed that the cyst nematodes isolated from Kobresia sp. and Kobresia humilis growing in Tianzhu alpine meadow steppe were H. avenae. This is the first report identifying Kobresia sp. and Kobresia humilis as hosts of H. avenae.

LI Hui-Xia, PENG Huan, PENG De-Liang, ZHU Rui-Dong, XU Peng-Gang, LI Jian-Rong. Identification of cyst nematode in alpine meadow steppe, Gansu[J]. Acta Prataculturae Sinica, 2015, 24(8): 174-180.

References

[1] Rivoal R, Cook R. Nematode pests of cereals. In: Evans K, Trudgill D L, Webster J M. Plant Parasitic Nematodes in Temperate Agriculture[M]. Wallingford, UK: CAB International, 1993: 259-303.
[2] Nicol J, Rivoal R, Vallette S, et al . The frequency and diversity of the cyst and lesion nematode on wheat in the Turkish Central Anatolian Plateau. Nematology, 2002, 4: 272.
[3] Roland N P, Maurice M. Plant Nematology[M]. London: CABI Publishing, 2006.
[4] Fisher J M, Hancock T W. Population dynamics of Heterodera avenae Woll. in South Australia. Australian Journal Agricultural Research, 1991, 42: 53-68.
[5] Chen P S, Wang M Z, Peng D L. A study of cereal cyst-nematode ( Heterodera avenae Wollenweber) in China. Acta Phytopathologic Sinica, 1992, 22(4): 339-342.
[6] Zhang D S, Peng D L, Qi S H. Factors influencing hatching of the cereal cyst nematode from the Northern Part of Huabei plain. Acta Phytopathologic Sinica, 1996, 26(2): 158.
[7] Nicol J, Rivoal R, Taylor S, et al . Global importance of cyst ( Heterodera spp.) and lesion nematode ( Pratylenchus spp.) on cereal: distribution, yield loss, use of host resistance and integration of molecular tools. Nematology Monographs, Perspectives, 2003, 2: 12-19.
[8] Yan G P, Smile R W. Distinguishing Heterodera filipjevi and H. avenae using polymerase chain reaction-restriction fragment length polymorphism and cyst morphology. Phytopathology, 2010, 100(3): 216-224.
[9] Yang C G, Wu H P, Tan G J, et al . Investigations on the distribution and damages caused by cereal cyst nematode in Anhui. Plant Protection, 2008, 34(2): 107-110.
[10] Chen X, Zhou H Y, Ma X. Distribution of Heterodera avenae in the middle and west regions of Inner Mongolia, China. Plant Protection, 2009, 35(5): 114-117.
[11] Peng D L, Huang W K, Sun J H, et al . First report of cereal cycst nematode ( Heterodera avenae ) in Tianjin, China. Nematology Research in China Vol. 4[M]. Beijing: Chinese Agricultural Science and Technology Press,2012.
[12] Li H X, Liu Y E, Wei Z, et al . The detection of Heterodera avenae from the cereal field in Autonomous regions of Tibet and Xinjiang. Nematology Research in China Vol.4[M]. Beijing: Chinese Agricultural Science and Technology Press, 2012.
[13] Peng D L, Ye W X, Gu X C, et al . First record of the cyst-forming nematode Heterodera filipjevi on wheat in China. Nematology Research in China Vol.3[M]. Beijing: Chinese Agricultural Science and Technology Press, 2010.
[14] Subbotin S A, Waeyenberge L, Moens M. Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNA-RFLPs. Nematology, 2000, 2(2): 153-164.
[15] Scholz U, Sikora A R A. Hatching behavior and life cycle of Heterodera latipons Franklin under Syrian agro-ecological conditions. Nematology, 2004, 6(2): 245-256.
[16] Qi S H, Peng D L, Zhang D S. Preliminary report of host and distribution area of cereal cyst nematode ( Heterodera avenae ) in China. Plant Protection, 1994, 20(4): 52.
[17] Wang M Z, Lei Z F, Xiao Y N. Host range of wheat cereal cyst nematode to some important plant. Plant Protection, 1996, 23(1): 2-4.
[18] Subbotin S A, Waeyenberge L, Molokanova I A, et al . Identification of Heterodera avenae group species by morphometrics and rDNA-RFLPs. Nematology, 1999, 1(3): 195-207.
[19] Peng D L, Subbotin S, Moens M. rDNA restriction fragment length polymorphism of Heterodera avenae in China. Acta Phytopathologic Sinica, 2003, 33(4): 323-329.
[20] Ou S Q, Peng D L, Li Y. rDNA-ITS restriction fragment length polymorphism of Qinghai, Shanxi and Henan province China. Acta Phytopathologic Sinica, 2011, 41(4): 411-420.
[21] Chen P S, Wang M Z, Peng D L. Preliminary report of identification on cereal cyst nematode of wheat in China. Scientia Agricultura Sinica, 1991, 24(5): 89-91.
[22] Zafar A H. Key and compendium to species of the Heterodera avenae group (Nematoda: Heteroderidae). Journal of Nematology, 2002, 34(3): 250-262.
[23] Wang M Z. Studies on a cyst nematode wheat disease I. identification of pathogen. Journal of Huazhong Agricultural University, 1991, 10(4): 352-356.
[5] 陈品三, 王明祖, 彭德良.我国小麦禾谷孢囊线虫 Heterodera avenae Wollenweber鉴定研究.植物病理学报, 1992, 22(4): 339-342.
[6] 张东升, 彭德良, 齐淑华. 华北平原北部禾谷孢囊线虫的孵化特点. 植物病理学报, 1996, 26(2): 158.
[9] 杨传广, 吴慧平, 檀根甲, 等. 安徽省小麦孢囊线虫田问分布及危害调查.植物保护, 2008, 34(2): 107-110.
[10] 陈新, 周洪友, 马玺. 内蒙古中西部地区小麦禾谷孢囊线虫的发生分布. 植物保护, 2009, 35(5): 114-117.
[11] 彭德良, 黄文坤, 孙建华, 等. 我国天津发现小麦禾谷孢囊线虫. 植物线虫学研究(第四卷)[M]. 北京: 中国农业科学技术出版社, 2012.
[12] 李惠霞, 柳永娥, 魏庄, 等. 新疆和西藏发现禾谷孢囊线虫的分布. 植物线虫学研究(第四卷)[M]. 北京: 中国农业科学技术出版社, 2012.
[13] 彭德良, 叶文兴, 顾晓川, 等. 我国发现菲利普孢囊线虫( Heterodera filipjevi )危害小麦.中国植物线虫学研究(第三卷)[M]. 北京: 中国农业科学技术出版社, 2010.
[16] 齐淑华, 彭德良, 张东升. 禾谷孢囊线虫在我国的新寄主及新分布区初报.植物保护, 1994, 20(4): 52.
[17] 王明祖, 雷智峰, 肖炎农. 小麦禾谷包囊线虫寄主范围研究. 植物保护, 1996, 23(1): 2-4.
[19] 彭德良, Subbotin S, Moens M. 小麦禾谷孢嚢线虫( Heterodera avenae )的核糖体基因(rDNA) 限制性片段长度多态性研究.植物病理学报, 2003, 33(4): 323-329.
[20] 欧师琪, 彭德良, 李玉. 青海、陕西部分地区禾谷孢囊线虫rDNA-ITS-RFLP的特征分析.植物病理学报,2011, 41(4): 411-420.
[21] 陈品三, 王明祖, 彭德良. 我国小麦禾谷孢囊线虫( Heterodera avenae Wollenweber)的发现与鉴定初报.中国农业科学, 1991, 24(5): 89-91.
[23] 王明祖.小麦孢囊线虫病的研究I:病原鉴定. 华中农业大学学报, 1991, 10(4): 352-356.
||