Screening, identification and assessment of endophytic bacteria antagonistic totomato bacterial spot

doi:10.11686/cyxb2014450

Abstract

Abstract: After screening by assessing inhibition zones on nutrient media an antagonistic endophytic bacteria, strain 264ZY7, was identified as having strong antagonistic ability against Pseudomonas syringe pv. tomato which causes bacterial spot on tomatoes. This strain was selected from 120 endophytic bacteria strains isolated from alpine meadow pasture. Strain 264ZY7 produced a 1.22 cm diameter inhibition zone. The bacteria is rod-shaped with a spore size of 1.534 μm×0.571 μm-3.210 μm×0.781 μm, gram-positive and was identified as Bacillus amyloliquefaciens based on morphology and homology analysis of the 16S rDNA and gyrB gene sequence. The control efficacy of strain 264ZY7 on bacterial spot on tomatoes grown in pots ranged up to 69%. It was also able to control fungal pathogens, in particular Fusarium sp. (52.84% efficacy). These results suggest that further development and utilization of this endophytic bacteria strain (264ZY7) should be undertaken.

FENG Zhong-Hong, WANG Yu-Qin, YANG Cheng-De*, XUE Li, CHEN Xiu-Rong. Screening, identification and assessment of endophytic bacteria antagonistic totomato bacterial spot[J]. Acta Prataculturae Sinica, 2015, 24(8): 166-173.

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