Partial replacement of chemical fertilizer by compound microbial inoculant and potential for promoting growth of intercropped Zea mays and Pisum sativum

doi:10.11686/cyxb20150203

Abstract

Abstract: In recent years, plant growth promoting rhizobacteria (PGPR) have become one of the most attractive strategies for the development of sustainable agricultural systems due to their eco-friendly nature, reduced consumption of non-renewable resources and lower cost. In order to investigate the effect of inoculant on growth and yield of intercropped Zea mays and Pisum sativum, five superior PGPR were screened by comprehensively analyzing nitrogenase activity, phosphate dissolving activity, 3-indoleacetic acid (IAA) secreting capacity and ability to antagonise pathogens. The compound microbial inoculant was produced by mixing these five strains with a rhizobia strain (ACCC 16101) from P. sativum. A field plot experiment was conducted to investigate the effects of the compound microbial inoculant on growth and yield of intercropped Z. mays and P. sativum. The viable count of compound microbial inoculant after 210 d storage was greater than 3.0×10⁹ cfu/mL, showing the quality of this inoculant complied with the standard for microbiological fertilizer of China (NY227-94). When the inoculant was applied in combination with 80% of the normal rate of chemical fertilizer, the root length of P. sativum at maturity was increased significantly (P<0.05) by 3.18 cm, and the root dry weight at full-bloom growth stage and maturity were increased by 0.88 and 2.39 g, respectively. The pod number per plant, seed number per pod, seed weight per plant and yield of P. sativum at maturity were increased significantly by 1.2 pods, 0.5 seeds, 0.63 g and 0.64 t/ha, respectively. The plant height of Z. mays at the flowering stage was increased (P<0.05) by 8.91 cm, while the cob weight and grain yield of Z. mays at maturity were increased significantly by 4.33 t/ha and 1.87 t/ha, respectively. From these data it can be calculated that applying the compound microbial inoculant to replace 20% of the chemical fertilizer normally used would increase the income from P. sativum by 972.00 yuan/ha and Z. mays by 3835.05 yuan/ha. Thus, partial fertilizer replacement with PGPR reduced the environmental impact of intercropped maize and peas by reducing consumption of non-renewable energy and protecting the agricultural environment, while also improving the food security.

RONG Liangyan, CHAI Qiang, YAO Tuo, ZHANG Rong, FENG Jin, YANG Hao, CAO Lei, ZHU Qian. Partial replacement of chemical fertilizer by compound microbial inoculant and potential for promoting growth of intercropped Zea mays and Pisum sativum[J]. Acta Prataculturae Sinica, 2015, 24(2): 22-30.

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