Proliferation stimulus and antioxidant effect of alfalfa flavonoids on dairy cow mammary epithelial cells cultured in vitro

doi:10.11686/cyxb2015030

Abstract

Abstract: This experiment studied the proliferation stimulus and antioxidant effects of different concentrations of alfalfa flavonoids on cow mammary epithelial cells cultured in vitro. Media with five different concentrations of alfalfa flavonoids were prepared to culture dairy cow mammary epithelial cells in vitro. Concentrations tested were: 0 μg/mL (control group), 25 μg/mL (trial group Ⅰ), 50 μg/mL (trial group Ⅱ), 75 μg/mL (trial group Ⅲ), 100 μg/mL (trial group Ⅳ). The results showed: 1) There was no significant difference in cell proliferation between the five concentrations during the first three days of cell culture, but the cell proliferation of trial groups Ⅱ-Ⅳ was significantly higher than for the control group and trial group Ⅰ (P<0.01) at day 5.2) The NO levels of trial groups Ⅱ-Ⅳ were significantly higher than the control group and trial group Ⅰ (P<0.01). Also, LDH activity of groups Ⅰ, Ⅱ, and Ⅳ was significantly lower than the control group and than test group Ⅲ (P<0.01), and the difference between the control group and test group Ⅲ was not significant. 3) Intracellular CAT content of trial group Ⅳ was significantly higher than the other groups (P<0.01). The MAD content of group Ⅲ was significantly lower than the other groups (P<0.01). GSH-PX activity of groups Ⅱ and Ⅲ was significantly higher than the other groups (P<0.01). In addition, there was no significant difference between the five groups in the cell SOD content. 4) The relative expression levels of p53, Caspase-3, SOCS3 and STAT1 genes in groups Ⅱ and Ⅲ were significantly lower than control group (P<0.01). In summary, alfalfa flavonoids can promote the proliferation of bovine mammary epithelial cells cultured in vitro, improve cell antioxidant capacity and inhibit cellular apoptosis. The best effect occurred at a dosage of 75 μg/mL of alfalfa flavonoids.

SU Xiao-Shuang, ZHAN Jin-Shun, ZHAN Kang, LIU Ming-Mei, ZHAO Guo-Qi. Proliferation stimulus and antioxidant effect of alfalfa flavonoids on dairy cow mammary epithelial cells cultured in vitro[J]. Acta Prataculturae Sinica, 2015, 24(12): 139-145.

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