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Acta Prataculturae Sinica ›› 2015, Vol. 24 ›› Issue (5): 159-166.DOI: 10.11686/cyxb20150519

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Comparison of four glyphosate tolerance genes

LIU Jian1, 2, **, XIAO Ya-Wen2, **, LU Jia1, 2, **, WU Zhong-Yi2, ZHANG Zhong-Bao2, XU Jie1, *, YAO Lei2, *   

  1. 1.College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China;
    2.Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry, Beijing 100097, China
  • Received:2014-05-16 Online:2015-05-20 Published:2015-05-20

Abstract: Glyphosate tolerance is an important agronomic trait in modern agriculture. In order to determine the best glyphosate tolerance gene for plant selectable markers we compared four glyphosate tolerance genes, cp4-epsps, GR79-epsps, gat4621 and GAT, in Arabidopsis. Four transgenic plants harboring each gene were treated with four different rates of glyphosate. The results showed that the resistance of plants with GAT gene was significantly higher than plants with the epsps gene. Plants with the gat4621 gene had the highest survival rate at all herbicide rates. The plants with the domestic GAT gene also had strong resistance. Reproductive development of Arabidopsis plants with the GAT gene was suppressed at higher glyphosate application rates.It was concluded that the GAT gene was more suitable as a selectable marker gene.