Effects of Epichloё endophyte and seed hydro-priming on the germination of Festuca sinensis under NaCl stress

doi:10.11686/cyxb2015143

Abstract

Abstract: This study investigated the germination and vigor of Festuca sinensis seeds infected (E+) and uninfected (E-) with Epichloё endophyte and under different levels of NaCl stress after hydro-priming. The results showed that NaCl stress treatments significantly reduced seed germination rate, interim germination, the germination index, the length of embryo and radicle and the dry weights of both E+ and E- F. sinensis seeds. Compared with E-, infection with Epichloё endophyte can significantly improve germination and seedling growth of F. sinensis seeds under different concentrations of NaCl. There were interaction effects between seed hydro-priming and the Epichloё endophyte. The highest levels of interaction were with 20 mins seed hydro-priming, when the germination indices and seedling growth rates were significantly higher than for all the other treatments. Correlation analysis showed that F. sinensis seed germination rate, interim germination, germination index, the length of embryo and radicle and dry weight were all significantly positively related to each other.

KUANG Yu, NAN Zhi-Biao, TIAN Pei. Effects of Epichloё endophyte and seed hydro-priming on the germination of Festuca sinensis under NaCl stress[J]. Acta Prataculturae Sinica, 2016, 25(2): 160-168.

References

[1] Umali D L. Irrigation-induced Salinity: a Growing Problem for Development and the Environment[M]. Washington: World Bank Publications, 1993.
[2] Chen L G. Sustainable development of agriculture and sustainable use of farm land resources in China. Journal of Anhui Agricultural Univerdity, 2001, 28(1): 102-105.
[3] Flowers T, Yeo A. Breeding for salinity resistance in crop plants: where next?. Functional Plant Biology, 1995, 22(6): 875-884.
[4] Flowers T, Troke P, Yeo A. The mechanism of salt tolerance in halophytes. Annual Review of Plant Physiology, 1977, 28(1): 89-121.
[5] Shi S L, Li J H. Ecological adaptability and aultivating techniques of two festuca Varieties. Acta Agretia Sinica, 2006, 14(1): 39-42.
[6] Siegel M, Latch G, Johnson M. Fungal endophytes of grasses. Annual Review of Phytopathology, 1987, 25(1): 293-315.
[7] White J F, Martin T I, Cabral D. Endophyte-host associations in grasses. XXII. Conidia formation by Acremonium endophytes on the phylloplanes of Agrostis hiemalis and Poa rigidifolia . Mycologia, 1996, 88(2): 174-178.
[8] Moy M, Belanger F, Duncan R, et al . Identification of epiphyllous mycelial nets on leaves of grasses infected by clavicipitaceous endophytes. Symbiosis, 2000, 28(4): 291-302.
[9] Dugan F, Sitton J, Sullivan R, et al . The Neotyphodium endophyte of wild barley ( Hordeum brevisubulatum subsp. violaceum ) grows and sporulates on leaf surfaces of the host. Symbiosis, 2002, 32(2): 147-159.
[10] Tadych M, Bergen M, Dugan F M, et al . Evaluation of the potential role of water in spread of conidia of the Neotyphodium endophyte of Poa ampla . Mycological Research, 2007, 111(4): 466-472.
[11] Bacon C W, Siegel M R. Endophyte parasitism of tall fescue. Journal of Production Agriculture, 1988, 1(1): 45-55.
[12] Schardl C L, Phillips T D. Protective grass endophytes: where are they from and where are they going?. Plant Disease, 1997, 81(5): 430-438.
[13] Sabzalian M R, Hatami B, Mirlohi A. Mealybug, Phenococcus solani , and barley aphid, Sipha maydis , response to endophyte-infected tall and meadow fescues. Entomologia Experimentalis et Applicata, 2004, 113(3): 205-209.
[14] Arachevaleta M, Bacon C, Hoveland C, et al . Effect of the tall fescue endophyte on plant response to environmental stress. Agronomy Journal, 1989, 81(1): 83-90.
[15] Buck D, Getz C, Guthman J. From farm to table: The organic vegetable commodity chain of Northern California. Sociologia Ruralis, 1997, 37(1): 3-20.
[16] Malinowski D P, Belesky D P. Adaptations of endophyte-infected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance. Crop Science, 2000, 40(4): 923-940.
[17] Reza Sabzalian M, Mirlohi A. Neotyphodium endophytes trigger salt resistance in tall and meadow fescues. Journal of Plant Nutrition and Soil Science, 2010, 173(6): 952-957.
[18] Nan Z B, Li C J. Roles of the grass- Neotyphodium association in pastoral agriculture systems. Acta Ecologica Sinica, 2004, 24(3): 605-616.
[19] Jin W J, Li C J, Nan Z B. Biological and physiologial charateristics of Neotyphodium endophyte symbiotic with Festuca sinensis . Mycosystema, 2009, (3): 363-369.
[20] Yang Y. Neotyphodium Endophyte in Festuca sinensis and Effect on Cold Tolerance to Host[D]. Lanzhou: Lanzhou University, 2010.
[21] Peng Q Q. Effect of Neotyphodium Endophyte on Chilling Tolerance to Festuca sinensis [D]. Lanzhou: Lanzhou University, 2012.
[22] Yao X, Li X Z, Zhu X X, et al . Effects of two fungicides on Neotyphodium seed-borne fungal endophyte of Festuca sinensis . Pratacultural Science, 2013, 30(10): 1517-1522.
[23] Peng Q Q, Li C J, Song M L, et al . Effects of seed hydropriming on growth of Festuca sinensis infected with Neotyphodium endophyte. Fungal Ecology, 2013, 6(1): 83-91.
[24] Wang Y R. Current status of seed priming reseach. Acta Prataculturae Sinica, 2004, 13(4): 7-12.
[25] Ma R X, Wang Y R. Advances in seed hydro-priming research. Acta Prataculturae Sinica, 2008, 17(6): 141-147.
[26] Li W, McDonald M, Bennett M, et al . Hydropriming of differing sized impatiens ‘Expo Wine’seeds. Seed Science and Technology, 2005, 33(3): 639-646.
[27] Taylor A G, Allen P S, Bennett M A, et al . Seed enhancements. Seed Science Research, 1998, 8(2): 245-256.
[28] Wang Y P, Wang Y X, Bai X L, et al . Effects of exogenous silicon on melon seed germination and the growth of seedling under NaCl stress. Acta Prataculturae Sinica, 2015, 24(5): 108-116.
[29] Wang Z F. Effects of Endophyte Infection on Salt Tolerance of Wild Barely ( Hordeum brevisubulatum )[D]. Lanzhou: Lanzhou University, 2009.
[30] Gou X Y. Effects of Neotyphodium Endophyte on Salt Tolerance to Drunken Horse Grass ( Achnatherum inebrians )[D]. Lanzhou: Lanzhou University, 2007.
[31] Zabalgogeazcoa I, Romo M, Keck E, et al . The infection of Festuca rubra subsp. pruinosa by Epichloё festucae. Grass and Forage Science, 2006, 61(1): 71-76.
[32] Song M L, Chai Q, Li X Z, et al . An asexual Epichloё endophyte modifies the nutrient stoichiometry of wild barley ( Hordeum brevisubulatum ) under salt stress. Plant and Soil, 2014, 387(1-2): 153-165.
[33] Ren A Z, Gao Y B, Zhang J, et al . Effect of endophyte infection on salt resistance of ryegrass. Acta Ecologica Sinica, 2006, 26(6): 1750-1757.
[34] Simpson W, Hume D. An examination of the potential mitigation effect of Neotyphodium infection on grasses exposed to elevated salt concentration[C]. Proceedings of the Fourth International Neotyphodium /Grass Interactions Symposium, Soest, Germany, 2000: 381-385.
[35] Ren A Z, Gao Y B, Li X. Effect of fungal endophyte infection on some physiological characters of Lolium perenne under drought conditons. Chinese Journal of Applied and Environmental Biology, 2002, 8(5): 535-539.
[36] Chen L. Effects of Hydro-Priming on the Germination and Emergence of Kentucky Bluegrass ( Poa pratensis ) Varieties[D]. Lanzhou: Lanzhou University, 2006.
[37] Jiang X W, Zhang W M, Yao D N, et al . Effect of priming on seed germination and vigor of Festuca arundinacea . Seed, 2008, 26(11): 14-21.
[38] Liu H X. Hydro-priming in Seed of Alfalfa ( Medicago sativa )[D]. Lanzhou: Lanzhou University, 2007.
[39] Carrillo G M, Bautista-Calles F, Villegas Monter A.Post harvest seed treatments to improve the papaya seed germination and seedlings development. Tropical and Subtropical Agroecosystems, 2013, 16(1): 133-141.
[40] Sun M, Yang Z T, Zhang C L, et al . Hydro-priming technique and its resistance physiogy effect for sea buckthorn seed. Scientia Silvae Sinicae, 2014, 50(12): 32-39.
[2] 陈利根. 中国农业可持续发展与耕地资源可持续利用. 安徽农业大学学报, 2001, 28(1): 102-105.
[5] 师尚礼, 李锦华. 羊茅属两种牧草生态适应性及其栽培技术. 草地学报, 2006, 14(1): 39-42.
[18] 南志标, 李春杰. 禾草-内生真菌共生体在草地农业系统中的作用. 生态学报, 2004, 24(3): 605-616.
[19] 金文进, 李春杰, 南志标. 中华羊茅内生真菌 Neotyphodium sp. 生物学与生理学特性的研究. 菌物学报, 2009, (3): 363-369.
[20] 杨洋. 中华羊茅内生真菌及其对宿主抗寒性的影响[D]. 兰州: 兰州大学, 2010.
[21] 彭清青. Neotyphodium 内生真菌对中华羊茅耐寒性的影响[D]. 兰州: 兰州大学, 2012.
[22] 姚祥, 李秀璋, 朱小晓, 等. 两种杀菌剂对中华羊茅种传内生真菌的影响. 草业科学, 2013, 30(10): 1517-1522.
[24] 王彦荣. 种子引发的研究现状. 草业学报, 2004, 13(4): 7-12.
[25] 马瑞霞, 王彦荣. 种子水引发的研究进展. 草业学报, 2008, 17(6): 141-147.
[28] 王玉萍, 王映霞, 白向利, 等. 硅对NaCl胁迫下甜瓜种子萌发及幼苗生长的影响. 草业学报, 2015, 24(5): 108-116.
[29] 王正凤. 内生真菌对野大麦耐盐性影响的研究[D]. 兰州: 兰州大学, 2009.
[30] 缑小媛. 内生真菌对醉马草耐盐性的影响研究[D]. 兰州: 兰州大学, 2007.
[33] 任安芝, 高玉葆, 章瑾, 等. 内生真菌感染对黑麦草抗盐性的影响. 生态学报, 2006, 26(6): 1750-1757.
[35] 任安芝, 高玉葆, 李侠. 内生真菌感染对黑麦草若干抗旱生理特征的影响. 应用与环境生物学报, 2002, 8(5): 535-539.
[36] 陈露. 水引发对草地早熟禾( Poa pratensis )萌发与出苗的影响[D]. 兰州: 兰州大学, 2006.
[37] 江绪文, 张文明, 姚大年, 等. 水引发处理对高羊茅种子萌发及活力的影响. 种子, 2008, 26(11): 14-21.
[38] 刘慧霞. 紫花苜蓿种子水引发研究[D]. 兰州: 兰州大学, 2007.
[40] 孙妙, 杨周婷, 张存莉, 等. 中国沙棘种子的水引发技术及其抗性生理效应. 林业科学, 2014, 50(12): 32-39.