Effects of RNAi expression of the ARF gene on enzyme activity at different developmental stages and storage temperatures in potato

doi:10.11686/cyxb2015194

Abstract

Abstract: ADP ribosylation factor (ARF) gene expression interference in a transformed potato cultivar “Gannongshu 2” was evaluated. Real time fluorescence quantitative polymerase chain reaction (qRT-PCR) showed that the relative expression of the ARF gene varied in the transgenic plants at different developmental stages and increased slightly at latter growth stages, indicating that ARF gene interference expression varied with maturity.Enzyme activity in the leaves was effected by ARF gene interference expression. For leaves of transgenic plants at different development stages, PPO activity increased by 11.61%-27.84%, NR activity increased by 21.10%-41.32%, PLD activity decreased 2.88%-57.64% SPS activity increased by 29.00%-39.57% when compared with the control. Relative expression of the ARF gene in stored tubers under different storage temperatures (room temperature and 4℃)showed the same trend; initially decreasing and then increasing. For transgenic tubers stored at room temperature, the activity of PPO increased by 30.44%-56.28%, NR activity increased by 17.41%-40.92%, PLD activity decreased 24.39%-85.11%, SPS activity increased by 30.89%-45.78% when compared with transgenic tubers stored at 4℃. For non-transgenic tubers stored at room temperature, PPO activity increased by 25.11%-70.66%, NR activity increased by 36.07%-89.62%, PLD activity decreased 11.35%-72.64% and SPS activity increased by 27.31%-61.33%.This study investigated the effect of RNAi expression of ARF gene on physiological and biochemical characteristics in potato to provide a theoretical basis for further study of the regulation role of the ARF gene in potato of different growth and stage of development.

ZHOU Xiang-Yan, ZHANG Ning, LIU Bai-Lin, PEI Rui-Fang, SI Huai-Jun, WANG Di. Effects of RNAi expression of the ARF gene on enzyme activity at different developmental stages and storage temperatures in potato[J]. Acta Prataculturae Sinica, 2016, 25(4): 133-139.

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