Abstract: The capacity for dissolved phosphorus absorption, the nitrogenase activity and the secretion of auxin of 19 strains of plant growth promoting rhizobacteria (PGPR) acquired from five plant species were tested by molybdenum blue colorimetry, acetylene reduction assay and high performance liquid chromatography, respectively. All 19 strains were tested for ability to antagonize the fungi Fusarium oxysporum f. sp. cucumerinum and Bipolaris sorokiniana, using the panel confrontation method. Strains with antagonistic ability were identified at morphological, physiological biochemical, and molecular levels. It was found that Bacillus subtilis strain LM4-3 had stronger nitrogenase activity than other tested strains and reached 244.88 nmol C₂H₄/(mL·h), while B. subtilis strain LHS11-1 had the highest dissolved phosphorus absorption capacity (205.77 mg/L), and the strain PGRS-3 had the greatest secretion of auxin, reaching 40.78 μg/mL in the culture medium. Five bacterial strains were found to have antagonistic activity against F. oxysporum f. sp. cucumerinum, with B. subtilis strain FX2-1 providing up to 69.07% suppression. and 51.73% of its fermentation solution. Seven bacterial strains could efficiently antagonize B. sorokiniana, with B. subtilis strain FX2-1 achieving 78.17%, the fermentation solution of B. subtilis LHS11-1 rinsed up to 81.52%. Other bacterial species found to have plant growth promoting or to be antagonistic towards the above two pathogenic fungal species included Bacillus cereus, Bacillus pumilus, Bacillus simplex and Pseudomonas fluorescens.