Transformation of ZjADH gene into Arabidopsis thaliana and cold-tolerance analysis of transgenic plants

doi:10.11686/cyxb2016158

Abstract

Abstract: To study the relationship between the ZjADH gene and plant cold tolerance, a plant expression vector 3302Y-ZjADH was constructed through DNA recombination technology. Transgenic Arabidopsis thaliana plants with glufosiante resistance were generated by floral dip method. PCR and qRT-PCR results showed that the target gene was successfully integrated with the genomes of Arabidopsis and could express efficiently. Both wild type and transgenic Arabidopsis plants were subjected to 4 ℃ to investigate their cold tolerance. Tests demonstrated that, compared with the wild type, the transgenic plants were more tolerant of cold stress. The free proline content in transgenic plants was significantly higher than the control, while the accumulation of ROS was much lower than the wild type. In addition, the expression levels of genes related to stresses like SOD, APX and LEA in transgenic plants were much higher than in the control. Results indicated that over-expression of ZjADH may improve the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and late embryogenesis abundant proteins (LEA) to improve the cold tolerance of transgenic plants. In conclusion, the heterologous expression of ZjADH genes could reinforce the resistance of plants to cold stress. This study provides a theoretical foundation for future research on the ZjADH gene and the cultivation of cold-tolerant Zoysia japonica cultivars.

ZHANG Lan, TENG Ke, XIAO Guo-Zeng, LIANG Xiao-Hong, XU Li-Xin, YIN Shu-Xia, CHAO Yue-Hui. Transformation of ZjADH gene into Arabidopsis thaliana and cold-tolerance analysis of transgenic plants[J]. Acta Prataculturae Sinica, 2016, 25(11): 43-49.

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