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Acta Prataculturae Sinica ›› 2017, Vol. 26 ›› Issue (12): 128-137.DOI: 10.11686/cyxb2017130

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Differential gene analysis of Poa pratensis in response to drought stress

LENG Nuan1, LIU Xiao-Wei2, ZHANG Na1, XU Li-Xin1, *   

  1. 1.Institute of Turfgrass Science, Beijing Forestry University, Beijing 100083, China;
    2.Nanjing Old Mountain Forest Farm, Nanjing 211811, China
  • Received:2017-03-21 Revised:2017-06-14 Online:2017-12-20 Published:2017-12-20
  • Contact: E-mail:lixinxu@bjfu.edu.cn

Abstract: Drought is one of the main factors affecting the productivity of Poa pratensis. This study was undertaken to reveal the change of gene expression profile of P. pratensis in the absence of a reference genome. The high-throughput Illumina Hiseq sequencing platform was used to investigate the transcriptome of a control group (CK) and a drought treated group (D) of P. pratensis. The sequencing data were subsequently analyzed to help reveal the molecular mechanisms of drought response in P. pratensis. The results showed that 24465 differentially expressed genes were detected under drought treatment. After screening 4143 up-regulated genes and 4415 down-regulated genes were obtained, accounting for 34.98% of the total number of differentially expressed genes. The genes related to protein kinase, protein phosphatase, carbon metabolism and ABA can be used as the main research object to study the drought response mechanism of P. pratensis. qRT-PCR analysis showed that the expression of 8 randomly differentially expressed genes was consistent with the high-throughput sequencing results. In addition, a series of genes for indole-3-glycerophosphate synthase, protein phosphatase, carbohydrate kinase, calcium binding protein and chlorophyll a/b binding protein were selected as candidate genes for drought stress related to P. pratensis. This laid the foundation for potentially revealing the molecular mechanism of drought response in P. pratensis.