Differential gene analysis of Poa pratensis in response to drought stress

doi:10.11686/cyxb2017130

Abstract

Abstract: Drought is one of the main factors affecting the productivity of Poa pratensis. This study was undertaken to reveal the change of gene expression profile of P. pratensis in the absence of a reference genome. The high-throughput Illumina Hiseq sequencing platform was used to investigate the transcriptome of a control group (CK) and a drought treated group (D) of P. pratensis. The sequencing data were subsequently analyzed to help reveal the molecular mechanisms of drought response in P. pratensis. The results showed that 24465 differentially expressed genes were detected under drought treatment. After screening 4143 up-regulated genes and 4415 down-regulated genes were obtained, accounting for 34.98% of the total number of differentially expressed genes. The genes related to protein kinase, protein phosphatase, carbon metabolism and ABA can be used as the main research object to study the drought response mechanism of P. pratensis. qRT-PCR analysis showed that the expression of 8 randomly differentially expressed genes was consistent with the high-throughput sequencing results. In addition, a series of genes for indole-3-glycerophosphate synthase, protein phosphatase, carbohydrate kinase, calcium binding protein and chlorophyll a/b binding protein were selected as candidate genes for drought stress related to P. pratensis. This laid the foundation for potentially revealing the molecular mechanism of drought response in P. pratensis.

LENG Nuan, LIU Xiao-Wei, ZHANG Na, XU Li-Xin. Differential gene analysis of Poa pratensis in response to drought stress[J]. Acta Prataculturae Sinica, 2017, 26(12): 128-137.

References

[1] Li W. Cloning and Expression Analysis of Transcription Factors Gene PpNAC in Poa pratensis L. Beijing: Chinese Academy of Forestry, 2011.
李伟. 草地早熟禾转录因子基因PpNAC的克隆和表达分析. 北京: 中国林业科学研究院, 2011.
[2] Xu L X, Han L B, Huang B R. Antioxidant enzyme qctivities and gene expression patterns in leaves of kentucky bluegrass in response to drought and post-drought recovery. Journal of The American Society for Horticultural Science, 2011, 136(4): 247-255.
[3] Xin J N. Genetic Transformation of Drought-resistance and Salt-resistance Gene in Kentucky Bluegrass ( Poa pratensis L.). Beijing: Beijing Forestry University, 2006.
信金娜. 草地早熟禾( Poa pratensis L.)抗旱耐盐基因遗传转化. 北京: 北京林业大学, 2006.
[4] Zhang Z B, Shan L. Advances in inheritance of crop drought resistance physiological traits. Chinese Science Bulletin, 1998, (17): 1812-1817.
张正斌, 山仑. 作物抗旱生理性状遗传研究进展. 科学通报, 1998, (17): 1812-1817.
[5] Wang X C, Yang Z R, Wang M, et al . High-throughput sequencing technology and its application. China Biotechnology, 2012, (1): 109-114.
王兴春, 杨致荣, 王敏, 等. 高通量测序技术及其应用. 中国生物工程杂志, 2012, (1): 109-114.
[6] Duhoux A, Carrere S, Gouzy J, et al . RNA-Seq analysis of rye-grass transcriptomic response to an herbicide inhibiting acetolactate-synthase identifies transcripts linked to non-target-site-based resistance. Plant Molecular Biology, 2015, 87(4/5): 473-487.
[7] Zhou Q, Luo D, Ma L C, et al . Development and cross-species transferability of EST-SSR markers in siberian wildrye ( Elymus sibiricus L.) using illumina sequencing. Scientific Reports, 2016, 6: 20549.
[8] Zhu C, Ai L, Wang L, et al . De Novo Transcriptome Analysis of Rhizoctonia Solani AG1 IA Strain Early Invasion in Zoysia japonica Root. Frontiers in Microbiology, 2016, 7: 708.
[9] Ahn J H, Kim J, Kim S, et al . De novo transcriptome analysis to identify anthocyanin biosynthesis genes responsible for tissue-specific pigmentation in Zoysia grass ( Zoysia japonica Steud.). Plos One, 2015, 10: e01379439.
[10] Zhao S, Zhang N, Liu D Y, et al . The impact of fenarimol application on the magnaporthe poae and fungi in the rhizosphere soil of Kentucky Bluegrass ( Poa pratensis L.). Journal of Nanjing Agricultural University, 2015, (4): 590-595.
赵爽, 张宁, 刘东阳, 等. 氯苯嘧啶醇施用对草坪斑枯病致病菌及根际土壤真菌的影响. 南京农业大学学报, 2015, (4): 590-595.
[11] Grabherr M G, Haas B J, Yassour M, et al . Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 2011, 29(7): 130-644.
[12] Anders S, Wolfgang H. Differential expression analysis for sequence count data. Genome Biology, 2010, 11(10): 106.
[13] Young M D, Wakefield M J, Smyth G K, et al . Gene ontology analysis for RNA-seq: accounting for selection bias. Genome Biology, 2010, 11: 142.
[14] Kanehisa M, Araki M, Goto S, et al . KEGG for linking genomes to life and the environment. Nucleic Acids Research, 2008, 36(SI): 480-484.
[15] Zhang J J, Li J J, Nian H J. The role of calcium/calmodulin signaling pathways in the stresses: progress in researches. Chinese Journal of Microecology, 2013, (7): 858-860.
张晶晶, 李金金, 年洪娟. 钙/钙调素信号途径在胁迫中的作用研究进展. 中国微生态学杂志, 2013, (7): 858-860.
[16] Sanchez-Barrena M J, Fujii H, Angulo I, et al . The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3. Molecular Cell, 2007, 26(3): 427-435.
[17] He L., Li F H, Sha L N, et al . Activity of serine/threonine protein phosphatase type-2C (PP2C) and its relationships to drought tolerance in maize. Acta Agronomica Sinica, 2008, (5): 899-903.
何亮, 李富华, 沙莉娜, 等. 玉米2C型丝氨酸/苏氨酸蛋白磷酸酶(PP2C)活性与耐旱性的关系. 作物学报, 2008, (5): 899-903.
[18] Bai X M. Cloning of MAPK gene which was activated by drought in Medicago sativa . Inner Mongolia Petrochemical Industry, 2011, (5): 18-20.
白雪梅. 紫花苜蓿( Medicago sativa )抗旱相关促分裂原活化蛋白激酶MAPK基因的克隆. 内蒙古石油化工, 2011, (5): 18-20.
[19] Ning Y S. Functional and Mechanistic Analysis of the SINA E3 Ligase OsDIS1 in Rice. Changsha: Hunan Agricultural University, 2011.
宁约瑟. 水稻SINA泛素连接酶OsDIS1的功能分析和作用机制研究. 长沙: 湖南农业大学, 2011.
[20] Kang Z L, Yang Y H, Zhang L J. Molecular mechanism of responsing to drought stress in plants. Journal of Maize Sciences, 2006, (2): 96-100.
康宗利, 杨玉红, 张立军. 植物响应干旱胁迫的分子机制. 玉米科学, 2006, (2): 96-100.
[21] Wang Y N, Liu C, Meng K, et al . Effects of exogenous carbon source on carbon and nitrogen metabolism of wheat under drought stress. Journal of Henan Agricultural Sciences, 2015, (10): 29-34.
王雅楠, 刘存, 孟珂, 等. 外源性碳源对干旱胁迫下小麦幼苗碳氮代谢的影响. 河南农业科学, 2015, (10): 29-34.
[22] Sasaki-Sekimoto Y, Taki N, Obayashi T, et al . Coordinated activation of metabolic pathways for antioxidants and defence compounds by jasmonates and their roles in stress tolerance in Arabidopsis . Plant and Cell Physiology, 2006, 47S: 233.
[23] Abebe T, Melmaiee K, Berg V, et al . Drought response in the spikes of barley: gene expression in the lemma, palea, awn, and seed. Functional & Integrative Genomics, 2010, 10(2): 191-205.
[24] Xu Y, Liu R, Yan L, et al . Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis . Journal of Experimental Botany, 2012, 63(3); 1095-1106.
[25] Pan L, Zhang X Q, Wang J P, et al . Transcriptional profiles of drought-related genes in modulating metabolic processes and antioxidant defenses in Lolium multiflorum . Frontiers in Plant Science, 2016, 7: 519.