Study of the semipermeable characteristics of Sorghum bicolor×Sorghum sudanense seeds

doi:10.11686/cyxb2017218

Abstract

Abstract: This study examines seed semipermeable properties and solutes leakage, investigating the testing of seed vigor by exploring the relationship between the presence of a semipermeable layer and electrical conductivity.In order to provide a scientific basis for seed quality evaluation, three Sorghum bicolor×Sorghum sudanense varieties were tested using measurements of water imbibition rate, electrical conductivity, tetrazolium salt uptake, and the fluorescent tracers technique. The results showed that the seed coat of Sorghum bicolor×Sorghum sudanense readily imbibed water, while showing poor permeability for tetrazolium salts and electrolytes. Intact seeds exhibited less strong staining with tetrazolium salt (5%, 0%, and 2% for Jicao No.2, Jicao No.8 and Jicao No.008, respectively) and there was no significant difference in the electrical conductivity of seeds with different germination rates (P>0.05). After puncturing, all seeds were strongly stained (100%) and electrical conductivity was significantly negatively correlated with germination rate (P<0.01). The seed coat permeability of the three Sorghum bicolor×Sorghum sudanense varieties to fluorescent tracers were different. Jicao No.8 and Jicao No.008 seed coats were not permeable to fluorescein. Lanthanum nitrate tracer combined with X-ray dispersive energy analysis revealed that a semipermeable layer was presented in the seed coverings of Sorghum bicolor×Sorghum sudanense. This is one of the main causes of differences in seed coat semipermeability, and its inhibition of electrolytes leakage resulted in the failure of electroconductivity tests to assess seed vigor.

Key words: Sorghum bicolor×Sorghum sudanense seed, semipermeability, semipermeable layer, seed vigor

SUN Qiu-jin, LÜ Yan-yan, HAN Yun-hua, WANG Yan-rong. Study of the semipermeable characteristics of Sorghum bicolor×Sorghum sudanense seeds[J]. Acta Prataculturae Sinica, 2018, 27(5): 162-169.

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