Effect of PEG priming on the cellular and mitochondrial structure and oxidation resistance of aged oat seed embryos

doi:10.11686/cyxb2017240

Abstract

Abstract: This study was designed to explore the effect of PEG priming on the seed germination, seedling growth, cellular and mitochondrial ultrastructure and oxidation resistance of aged oat (Avena sativa) seed embryos. Seeds with ultra-dry moisture content (4%) were deteriorated for 48 d at 45 ℃, and primed with either 0 (distilled water) or -1.2 MPa of PEG-6000 for 12 h at 20 ℃ under darkness conditions. Analysis was then undertaken to determine the seed germination percentage, germination index, mean germination time, seedling vigor index, cellular and mitochondrial ultrastructure and their superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR) and malondiadehyde (MDA). The results showed that the activities of cellular and mitochondrial SOD, APX and GR were increased by PEG priming in the embryo of ultra-dried and aged oat seeds. The activities of cellular SOD, APX and GR increased by 26.7%, 20.9% and 26.9% respectively. The activities of mitochondrial SOD, APX and GR increased by 19.2%, 19.4% and 17.1%. MDA contents declined by 28.3% and 13.7%, maintaining cellular and mitochondrial structures and hence promoting seed germination and seedling growth. However, the opposite changes were observed in seeds tested with distilled water priming. The ultrastructure and oxidation resistances showed a close and collaborative relationship between embryonic cells and their mitochondria in ultra-dried and aged oat seeds during the priming process, with the role of mitochondria more important than that of the embryonic cells.

Key words: oat, cell, mitochondria, seed ageing, PEG priming

XIA Fang-shan, DONG Qiu-li, MAO Pei-sheng, WANG Ming-ya, CHEN Ling-ling, CHENG Hang. Effect of PEG priming on the cellular and mitochondrial structure and oxidation resistance of aged oat seed embryos[J]. Acta Prataculturae Sinica, 2018, 27(5): 170-177.

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