Effect of combined applications of selenium and zinc on herbage yield, Zn, Se accumulation and amino acid content of alfalfa

doi:10.11686/cyxb2018185

Abstract

Abstract: This experiment investigated the effect of added zinc (Zn) and selenium (Se) in calcareous soils with severe Zn and Se deficiencies, on alfalfa yield and herbage Zn, Se, and amino acid content in a pot experiment. The design was a 3×3 complete factorial with three levels of Zn (0, 0.10, 0.40 g·kg^-1), and three levels of Se, also (0, 0.30, 1.80 mg·kg^-1). A comprehensive evaluation of alfalfa herbage amino acid composition as indicated by the amino acid score (AAS), chemical score (CS) and essential amino acid index (EAAI), was also undertaken. It was found that all treatments expect for Zn alone at 0.10 g·kg^-1 significantly increased alfalfa herbage yield. Se alone provided a greater increase in alfalfa herbage yield than Zn alone, and combined Se-Zn applications had the best effect. Similarly, alfalfa Se content was significantly increased by Se application, with combined application of Se and Zn showing a synergistic effect. Application of Zn significantly increased alfalfa Zn content, and combined application of Zn and Se at the lower rate promoted Zn absorption, whereas Se application at the higher rate was not conducive to Zn absorption. The total amino acid content of alfalfa herbage ranged from 15.88-18.89 g·100 g^-1, with the Se₁Zn₁ treatment having the highest value, and the Se₂Zn₂ treatment having the lowest. Meanwhile essential amino acid content of alfalfa herbage ranged from 5.11-6.45 g·100 g^-1, with the Se₁Zn₂ treatment having the highest values, and the Se₂Zn₂ treatment having the lowest value. The treatments Se₁Zn₀, Se₂Zn₁, Se₀Zn₂ and Se₁Zn₂ all had an essential∶total herbage amino acid value close to 40%. The first limiting amino acids in all treatments were the sulfur-containing amino acids (methionine+cystine). The results obtained in this study suggest that in the severely Se and Zn deficient calcareous soil tested, the optimal Zn application rate was 0.1-0.4 g·kg^-1 soil, and the optimal Se application rate was 0.3 mg·kg^-1 soil. These rates not only improved alfalfa herbage yield, but also improved amino acid composition and quality.

Key words: alfalfa, selenium (Se), zinc (Zn), amino acids, nutritional value

TIAN Chun-li, LI Bin, LIU Fang, ZHAO Ying, LIU Shi-liang, JIE Xiao-lei, HU Hua-feng. Effect of combined applications of selenium and zinc on herbage yield, Zn, Se accumulation and amino acid content of alfalfa[J]. Acta Prataculturae Sinica, 2019, 28(3): 142-153.

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