Effects of different rhizobium fertilizers on alfalfa productivity and soil fertility

doi:10.11686/cyxb2019344

Abstract

Abstract: After potting and field experiments to screen for the best match with Medicago sativa cv. Gannong No.3 and Gannong No.9, six rhizobium strains (Da99, DL58, G3G2, Ga66, QL31B and Wa32) were selected for further research and prepared as solid rhizobium fertilizers. In April 2017, the rhizobium fertilizers were tested on a second-year stand of M. sativa cv. Gannong No.5, to study their effect on plant productivity and soil fertility in an alfalfa crop. Non-fertilizer was used as control. It was found that strain Da99 produced the greatest effects on plant dry weight and height (increases of 41.75% and 9.30%, respectively; P<0.05), compared with the control. However, none of the rhizobium fertilizers significantly affected the stem∶leaf ratio. The strains QL31B, DL58 and G3G2, significantly lowered water content of the air-dried soil, compared to the control. Differences rhizobium fertilizer reduced the soil pH. Treatment with Wa32 increased soil organic matter, alkaline nitrogen and available phosphorus contents by 75.39%, 22.79% and 76.63%, respectively, compared to the control. Treatment with Da99 and G3G2 strains significantly increased soil available potassium levels by 27.35% and 24.03%, respectively, compared to the control. Some rhizobium fertilizer treatments improved soil urease activity, but effects on soil catalase activity were not significant. Treatment with QL31B increased the soil sucrase and alkaline phosphatase activities by 76.94% and 21.81%, respectively, compared to the control. The soil cellulase activity after treatment of G3G2 and Ga66 was increased significantly by 22.35% and 18.82%, respectively, compared to the control. Multivariate evaluation showed that treatment with the six rhizobium fertilizers significantly improved the productivity and soil fertility of alfalfa. The best performing rhizobial strain, with the most obvious effect on plant growth and soil fertility in the current study, was QL31B, which is therefore preliminarily concluded to be a broad-spectrum rhizobium.

Key words: alfalfa, rhizobium, productivity, soil fertility, grey correlation degree

HE Guo-xing, SONG Jian-chao, WEN Ya-jie, LIU Cai-ting, QI Juan. Effects of different rhizobium fertilizers on alfalfa productivity and soil fertility[J]. Acta Prataculturae Sinica, 2020, 29(5): 109-120.

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