Responses of AMF and PGPR to Festuca elata under phenanthrene and pyrene stress

doi:10.11686/cyxb2018510

Abstract

Abstract: The purpose of the present study was to evaluate mechanisms (and their efficiency) for degradation of residues of phenanthrene and pyrene by arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR). A total of 16 treatments were designed involving Festuca elata, with or without inoculation with AMF Glomus versiforme (Gv), PGPR Pseudomonas fluorescens (Ps2-6), or Gv+Ps2-6, and grown soil with 0 (Control), 50, 100 or 150 mg·kg^-1 added polycyclic aromatic hydrocarbons (PAHs) (phenanthrene∶pyrene=1∶1 in weight). The results showed that inoculation with AMF, PGPR or AMF+PGPR significantly increased the biomass and mycorrhizal infection rate of tall fescue, enhanced photosynthesis and chlorophyll content of plants, and significantly increased the physiological activity of plants. At 100 mg·kg^-1 phenanthrene and pyrene, the chlorophyll a, b and total chlorophyll content in the leaves of tall fescue were increased by 57.7%, 41.7% and 51.8%, respectively, compared with the Control, and the net photosynthetic rate (P_n), transpiration rate (T_r), intercellular CO₂ concentration (C_i) and gas pore conductivity (G_s) were increased by 70.6%, 100.0%, 4.5% and 78.6%, respectively; the maximum photochemical efficiency (F_v/F_m) and PSⅡ potential activity (F_v/F_o) were increased by 2.2% and 8.8%, respectively. The activities of superoxide dismutase (SOD), catalase (CAT), soluble sugar and proline were, respectively, 1.6, 1.5, 2.3 and 2.7 times those of the Control; the malondialdehyde content was decreased by 46.0%, and the height and fresh weight were increased by 63.0% and 69.6%. Inoculation with PGPR increased AMF infection rate and mycorrhizal dependency. Under the test conditions, double inoculation with a Gv+Ps2-6 treatment increased the content of photosynthetic pigments and antioxidant capacity of Festuca elata, enhanced photosynthetic capacity, reduced the level of membrane lipid peroxidation, and significantly promoted plant growth.

Key words: arbuscular mycorrhizal fungi, plant growth-promoting rhizobacteria, phenanthrene and pyrene, photosynthesis, chlorophyll, physiology

LI Wen-bin, NING Chu-han, LI Wei, LI Feng, GUO Shao-xia. Responses of AMF and PGPR to Festuca elata under phenanthrene and pyrene stress[J]. Acta Prataculturae Sinica, 2019, 28(8): 84-94.

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