Dynamic effects of exogenous fibrolytic enzyme supplementation on nutritive value, fermentation quality and enzyme activities of fermentation total mixed ration

doi:10.11686/cyxb2018602

Abstract

Abstract: The objective of this study was to determine the effect of exogenous fibrolytic enzyme (EFE) supplementation in fermentated total mixed ration (FTMR) on the nutritional value, fermentation quality and enzyme activities, and to find out the optimal level of EFE addition. A completely randomized experimental design was adopted, and 5 levels of EFE (0, 0.4, 0.8, 1.6 and 3.2 g·kg^-1 dry matter) were added to FTMR as the 5 experimental treatments. At 7, 14, 35, 49, 70 days after initiation of fermentation, major nutrients, water soluble carbohydrate (WSC), fermentation parameters and activities of four enzymes were determined. It was found: 1) With increased fermentation time, the dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF), and WSC contents and the activities of the four enzymes monitored showed a decrease (P<0.05), while acetic acid accumulated with time (P<0.05). 2) With increased EFE supplementation, the DM, NDF, ADF and WSC contents, pH value, ammonia nitrogen content, and the activities of carboxyl peptidase and acid protease decreased (P<0.05) until day 35 and then stabilised, while the acetic acid content, and cellulase and xylanase activities increased and stabilized over the same time period (P<0.05). At day 35, the EFE supplementation treatments of 0.8 and 1.6 g·kg^-1 showed lower pH values, NDF and ADF contents, and higher activity of cellulase and xylanase (P<0.05). It was found that the addition of EFE promoted fiber degradation and the accumulation of organic acids through enzymatic activity, inhibited the activities of carboxypeptidase and acidic protease, and reduced the loss of protein in FTMR. The dose effect was additive and the optimum EFE dose was 0.8-1.6 g·kg^-1 DM.

Key words: FTMR, exogenous fibrolytic enzymes, enzyme activity, dynamic effects

CHEN Guang-ji, WU Jia-hai, SHANG Yi-shun, ZHANG Rong, HE Ting-zhang, WU Yan, LOU Fen, XIONG Jun, GAN Xiao-bo, CHEN Yin-qiong, WANG Pu-chang, HAN Yong-fen, LI Shi-ge, SHU Jian-hong. Dynamic effects of exogenous fibrolytic enzyme supplementation on nutritive value, fermentation quality and enzyme activities of fermentation total mixed ration[J]. Acta Prataculturae Sinica, 2019, 28(9): 123-134.

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