Effects of antioxidant priming on germination and seedling growth of aged seeds of smooth bromegrass

doi:10.11686/cyxb2018788

Abstract

Abstract: The poor vigor of aged seeds is a technical problem that needs to be solved for the protection of plant germplasm resources and for agricultural production. In this study, an antioxidant priming technique was used to treat smooth bromegrass seeds aged for 16 days, to determine the suitable antioxidant and optimum priming conditions to postpone seed aging. Aged seeds were conditioned with ascorbic acid (AsA, 2 mmol·L^-1), glutathione (GSH, 0.25 mmol·L^-1), melatonin (MT, 100 μmol·L^-1), or water (WT), and then germination and seedling growth characteristics, and the activities of superoxide dismutase, peroxidase, and catalase were determined. There were differences in effectiveness among the priming treatments. All the priming treatments significantly (P<0.05) increased the germination percentage, germination potential, germination index, vigor index, leaf length, root length, and seedling weight, and significantly (P<0.05) decreased the mean germination time. Compared with WT, priming with antioxidants significantly increased the vigor index and seedling weight of aged seeds. Among the three antioxidants, GSH significantly (P<0.05) promoted the root length of seedlings, germination percentage, and germination potential, and resulted in the highest values for germination percentage and germination potential. Priming with water had no significant (P>0.05) effect on antioxidant enzyme activity in aged seeds. However, compared with water priming, antioxidant priming of aged seeds significantly (P<0.05) enhanced seed peroxidase activity, and priming with AsA and GSH significantly (P<0.05) increased seed catalase activity. The data for the eight indexes of germination and seedling growth in the priming treatments were subjected to a cluster analysis. This analysis showed that GSH had the strongest positive effect on the seed germination and seedling growth of smooth bromegrass. A principal component analysis showed that, of all the indexes, the germination percentage and germination potential best reflected the effect of priming on germination and seedling growth of aged seeds. Therefore, antioxidant priming, especially with GSH, can significantly enhance the vigor of aged smooth bromegrass seeds, although the repair of aging damage can take some time.

Key words: seed aging, seed priming, ascorbic acid, glutathione, melatonin, smooth bromegrass

SUN Ming, WANG Si-qi, Aierken· Dawuti, MAO Pei-sheng. Effects of antioxidant priming on germination and seedling growth of aged seeds of smooth bromegrass[J]. Acta Prataculturae Sinica, 2019, 28(11): 105-113.

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