Effects of composite exogenous hormone application on induction of systemic resistance to Rhizoctonia solani in creeping bentgrass

doi:10.11686/cyxb2018255

Abstract

Abstract: Composite plant hormone formulations with different concentrations of epibrassinolide (EBR), salicylic acid (SA) and ethephon (ET) were applied exogenously to “Penn-A4” bentgrass with induction times of 5, 7, and 10 days to test if the hormone treatments could enhance disease resistance. The effect of the exogenous composite hormone application and induction time treatments on the brown spot resistance of creeping bentgrass was assessed by determining a disease index, the control efficacy, antioxidant enzyme activity and ascorbic acid/glutathione cycle changes. Some treatments did improve the disease resistance of creeping bentgrass. The disease index for an ET+EBR hormone treatment with 7 days induction was significantly lower than other treatments, and the anti-disease effect was quantified at 40.51%. Moreover, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities increased by 130.53%, 197.40% and 101.66%, respectively, while ascorbate peroxidase (APX), dehydroascorbate reduction (DHAR), and glutathione reductase (GR) activities increased by 316.26%, 41.95% and 80.34%, respectively, compared with the control, after this treatment. In addition, active deoxyascorbic acid (AsA), oxidized glutathione (GSH) content, and AsA/hydroascorbic acid (DHA), and GSH/reduced glutathione (GSSG) ratios were all higher for the ET+EBR combination, than for the control and other treatments. In summary, exogenous application of mixtures of EBR, SA and ET were able to induce brown spot resistance in creeping bentgrass, and an ET+EBR combination with an induction period of 7 days had the best effect. The results of this study provide technical information for the development and promotion of novel hormone-based agents for induction of plant disease resistance.

Key words: creeping bentgrass, composite exogenous hormone, Rhizoctonia solani, induced resistance, antioxidant enzymes, ascorbate-glutathione cycle

ZHAO Chun-xu, JIANG Han-yu, DONG Wen-ke, CHEN Hong, FANG Yan-xia, XIE Li-ping, MA Hui-ling. Effects of composite exogenous hormone application on induction of systemic resistance to Rhizoctonia solani in creeping bentgrass[J]. Acta Prataculturae Sinica, 2018, 27(11): 120-130.

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