Construction of a genetic linkage map for tetraploid potato based on SRAP markers

doi:10.11686/cyxb2019230

Abstract

Abstract: This research aimed to lay a foundation for molecular assisted breeding of potato through location of quantitative trait loci (QTL) for important traits such as high starch content, high dry matter content and yield of potato tubers. The mapping population comprised 182 F₁ genotypes from a cross of YSP-4×MIN-021 tetraploid hybrid potato lines. We constructed biparental genetic linkage maps based on sequence related amplified polymorphism (SRAP) molecular makers. A total of 36 out of 357 suitable SRAP primers were selected, the genomic DNA of the 182 F₁ genotypes and their parents was amplified, and a total of 598 SRAP markers was obtained. The chi-square test showed that there were 127 distorted markers, of which 59 were in the maternal linkage group, 68 were in the paternal linkage group, and the segregation distortion ratio was less than 30%, which conformed to the requirements for genetic mapping. The two parental SRAP genetic maps of tetraploid potato were established using the software package Join Map 4.0. The maternal map included 274 markers, had a total length of 1572.2 cM with an average interval of 5.74 cM, and the length of the linkage groups ranged from 14.03 cM to 214.44 cM. The paternal map included 324 markers, had a total length of 1932.23 cM with an average interval of 5.96 cM, and the length of the linkage groups ranged from 93.65 cM to 242.06 cM.

Key words: tetraploid potato, SRAP markers, genetic linkage map

ZHANG Ming-fei, YU Zhuo, YU Xiao-xia, LI Jing-wei, MA Yan-hong, WU Guo-fang, WANG Ying. Construction of a genetic linkage map for tetraploid potato based on SRAP markers[J]. Acta Prataculturae Sinica, 2019, 28(8): 190-199.

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