Incidence of oat leaf spot and pathogen identification in the main oat production areas of Gansu Province

doi:10.11686/cyxb2019304

Abstract

Abstract: This study aimed to clarify the occurrence and pathogenic species causing oat leaf spot in the main oat producing areas of Gansu Province, and provide a theoretical foundation for prevention and control of this disease. Occurrence of oat leaf spot was investigated, and leaves were collected from the field for isolation, culture, pathogenicity testing, and pathogen identification. Collection locations included Tongwei County of Dingxi City, Shandan and Minle Counties of Zhangye City, Tianzhu County of Wuwei City, and Gannan County of Hezuo City. Regional differences in the occurrence of oat leaf spot were found. The occurrence was the most serious in Tongwei County of Dingxi City, where the mean leaf incidence was 96.15% and the average disease index was 70.26. Moderate leaf spot incidence and disease index (95.07% and 69.16, respectively) were recorded in Tianzhu County of Wuwei City. A relatively light occurrence was observed in Minle County of Zhangye City, where the leaf incidence was 8.78%, and the disease index was 2.45. Disease symptoms induced by the isolated and purified fungi were the same as the symptoms in the field after the pathogenicity test, and the same fungi could be obtained after re-isolation. Further morphological observation and rDNA-ITS sequence analysis indicated that a total of 10 pathogens caused oat leaf spot in the different localities where samples were collected. These were Drechslera avenae, Pyrenophora avenae, Pyrenophora chaetomioides, Alternaria alternata, Alternaria brassicae, Alternaria mali, Bipolaris sorokiniana, Cladosporium cladosporioides, Cladosporium herbarum and Epicoccum nigrum. The dominant fungi were D. avenae and A. alternata. This study clarified the occurrence and identity of pathogenic species causing oat leaf spot in Gansu Province. With the exception of D. avenae and P. avenae, the identified pathogens have, to our knowledge, not previously been reported as causes of domestic oat leaf spot.

Key words: oat leaf spot, disease investigation, pathogenic identification

NIE Xiu-mei, ZHAO Gui-qin, SUN Hao-yang, CHAI Ji-kuan, LAN Xiao-jun, ZHOU Heng, LI Rong, JU Ze-liang, JIAO Run-an, SUN Lei-lei. Incidence of oat leaf spot and pathogen identification in the main oat production areas of Gansu Province[J]. Acta Prataculturae Sinica, 2020, 29(4): 157-167.

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