An analysis of the response of the LpWRKY20 gene to abiotic stress and its role in drought resistance

doi:10.11686/cyxb2019318

Abstract

Abstract: This research investigated the expression of the LpWRKY20 gene under different abiotic stresses, using quantitative reverse transcription PCR (qRT-PCR). The plant expression vector pBI121-LpWRKY20-GFP was transferred into tobacco by the leaf disc method and transgenic lines were obtained. The expression of this gene differed under different abiotic stresses. The phenotype of transgenic plants was better than that of wild plants under drought stress. The activities of superoxide dismutase and catalase of transgenic plants were higher than those of the wild type, and the ability to remove free radicals in vivo increased significantly over time. Tissue malondialdehyde level was lower than that of the wild type, indicating that the cell membrane of transgenic plants was less damaged and had a strong self-repairing ability. The change of phenotype and physiological indexes under drought conditions indicated that transgenic plants had strong drought resistance, and the preliminary conclusion was reached that the LpWRKY20 gene confers drought resistance.

Key words: Lilium pumilum, LpWRKY20, abiotic stress, genetic transformation, drought resistance

YANG Liu-hui, YIN Hang, HUANG Qin-mei, ZHANG Yan-ni, HE Miao, ZHOU Yun-wei. An analysis of the response of the LpWRKY20 gene to abiotic stress and its role in drought resistance[J]. Acta Prataculturae Sinica, 2020, 29(1): 193-202.

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