Autotetraploid plant induction and the genome size estimation in Lanzhou lily (Lilium davidii var. unicolor)

doi:10.11686/cyxb2019425

Abstract

Abstract: Lanzhou lily (Lilium davidii var. unicolor, 2n=2x=24) is a well-known plant that is used both as food and for medicinal purposes. It is of great significance to breed elite germplasm and estimate its genome size. Autotetraploid induction in Lanzhou lily was accomplished in this study by soaking in different concentrations of colchicine, using seeds and tube-bulbs as the explants. Numbers of autotetraploids identified by flow cytometry assay among the plants derived from colchicine treated seeds and tube-bulbs were 10 and 4, respectively. The tetraploid induction rates were 21.11% for seeds and 33.27% for tube-bulbs. In order to estimate the genome size of Lanzhou lily, Triticum aestivum “Chinese spring” was used as an internal standard. The DNA content of both diploid and tetraploid Lanzhou lily plants was tested by flow cytometry. The genome size of Lanzhou lily was found to be 64.9 Gbp, four times greater than that of T. aestivum “Chinese spring”.

Key words: Lanzhou lily, colchicine, autotetraploid, flow cytometry, genome size

LI Shu-jie, ZHANG Jin-wen, PEI Huai-di, WANG Hong-mei, LUO Jun-jie, LIN Yu-hong. Autotetraploid plant induction and the genome size estimation in Lanzhou lily (Lilium davidii var. unicolor)[J]. Acta Prataculturae Sinica, 2020, 29(3): 190-196.

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