Effects of dietary concentration:roughage ratio on rumen fermentation and flora population structure in Hu sheep

doi:10.11686/cyxb2019435

Abstract

Abstract: To study the effects of dietary concentration:roughage ratio on fermentation and flora population structure in Hu sheep, forty healthy lambs with similar body weight and good body condition were randomly divided into four groups with ten sheep per group (5 male and 5 female). Sheep in each group were fed total mixed ration with concentration:roughage ratio of 70:30 (A), 60:40 (B), 50:50 (C) or 40:60 (D), respectively. The experimental period was 70 days containing a 14-day pre-experiment adaptation period and a 56-day experiment. It was found that: 1) The concentrations of ammonia nitrogen, propionic acid, butyric acid and valeric acid in group B were significantly higher than those of group D (P<0.05). 2) In total, 875 operational taxonomic units (OTUs) were detected, and 725 of these OTUs (82.86% of total OTUs) were shared by all four treatment groups. Group A possessed six unique OTUs, while group D had one unique OTU. The number of phyla, and Shannon diversity index were significantly higher in group A than in groups C and D (P<0.05), while the Simpson index was significantly lower in group A than that of groups B and C (P<0.05).The bacterial diversity in group C was significantly different from that in group A and group B (P<0.05). 3) At the phylum level, the relative abundance of Actinobacteria, and Spirochaetae in group A was significantly higher than the abundance in groups C and D (P<0.05). At the genus level, the relative abundance values for Lachnospiraceae_ND3007, Treponema_2, Weissella, Anaeroplasma, Brevibacterium, Pseudobutyrivibrio, an uncultured rumen bacterium and Roseburia were significantly higher in group A than in group D (P<0.05). The relative abundance of Lachnospiraceae_XPB1014 and Uncultured_Bacteroidales_BS11 in group A was significantly lower than those of group C and D, respectively (P<0.05). In conclusion, a high concentration:roughage ratio enhanced microbial richness and diversity in rumen of Hu sheep, and thus promoted rumen fermentation.

Key words: Hu sheep, total mixed ration, rumen fermentation, microbial community structure

ZHAN Jin-shun, YANG Qun, HU Yao, WU Yan-ping, HUO Jun-hong. Effects of dietary concentration:roughage ratio on rumen fermentation and flora population structure in Hu sheep[J]. Acta Prataculturae Sinica, 2020, 29(7): 122-130.

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