The species diversity and community assembly of arbuscular mycorrhizal fungi in typical alpine grassland in Sanjiangyuan region

doi:10.11686/cyxb2020268

Abstract

Abstract:

This study investigated the key processes driving arbuscular mycorrhizal （AM） fungal community changes in typical alpine grassland ecosystems， by systematically measuring AM fungal diversity and community composition in four typical grassland ecosystems of Sanjiangyuan National Park， based on the Illumina-Miseq high-throughput sequencing method. The ecosystems evaluated were： Alpine desert， alpine steppe， alpine meadow and alpine wetland. Ecological processes structuring the fungal communities were inferred according to phylogenetic patterns and species abundance distributions in different ecosystems. It was found that： 1） The dominant genus in alpine desert， alpine meadow and alpine wetland was Glomus， while the dominant genus in alpine steppe was Diversispora. 2） The OTU richness and phylogenetic diversity index of AM fungi in alpine wetland were significantly lower than those of the other three grassland ecosystem types. 3） There were significant differences in species composition and phylogenetic composition of AM fungal communities among different grassland types. Plant community composition， soil water content， and available N∶P were the main factors affecting the composition of the AM fungal community. Soil water content was the primary determinant of the species composition of the AM fungal community， and plant community composition was a key factor in determining the phylogenetic composition of the AM fungal community. 4） AM fungal communities were all phylogenetically clustered in the four grassland types. The phylogenetic structure of AM fungal communities in the different grassland types are clustered. In alpine deserts， AM fungal community assembly is determined by stochastic processes， while in alpine grasslands， alpine meadows and alpine wetlands， AM fungal community assemblage are determined by stochastic processes and weak responses to environmental filtering.

Key words: alpine grassland ecosystem, arbuscular mycorrhizal （AM） fungi, phylogenetic structure, community assembly

Cong-cong LI, Ya-xing ZHOU, Qiang GU, Ming-xin YANG, Chuan-lu ZHU, Zi-yuan PENG, Kai XUE, Xin-quan ZHAO, Yan-fen WANG, Bao-ming JI, Jing ZHANG. The species diversity and community assembly of arbuscular mycorrhizal fungi in typical alpine grassland in Sanjiangyuan region[J]. Acta Prataculturae Sinica, 2021, 30(1): 46-58.

Figures/Tables 11

References 72

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变量 Variables	高寒荒漠 Alpine desert	高寒草原 Alpine steppe	高寒草甸 Alpine meadow	高寒湿地 Alpine wetland	变异系数 Coefficient of variation (CV, %)
植物丰富度Plant richness	8.54±0.45ab	7.92±0.21bc	9.54±0.65a	6.78±0.78c	24
香农-威纳指数Shannon-Wiener index	1.93±0.05a	1.89±0.03a	2.06±0.09a	1.65±0.11b	14
土壤含水量SWC （%）	4.39±0.44d	14.06±0.38c	22.60±1.03b	37.30±2.82a	68
土壤pH Soil pH	8.88±0.02a	8.18±0.02b	8.07±0.05b	8.19±0.09b	4
土壤有机碳SOC (g·kg^-1)	5.83±0.85c	27.68±5.10b	58.75±3.55a	53.19±5.51a	73
可溶性有机碳DOC (mg·kg^-1)	68.04±1.63c	119.51±6.46b	160.34±5.16a	156.45±4.17a	33
土壤全氮TN (g·kg^-1)	0.75±0.07c	2.84±0.46b	5.00±0.35a	5.28±0.43a	66
土壤全磷TP (g·kg^-1)	0.25±0.01b	0.51±0.02a	0.51±0.02a	0.49±0.03a	29
土壤有效氮AN (mg·kg^-1)	11.48±0.50c	58.84±2.12b	78.15±5.22a	73.57±11.68a	60
土壤有效磷AP (mg·kg^-1)	2.62±0.46c	6.39±0.44b	8.80±0.42a	5.80±0.90b	49
有效氮磷比N∶P	5.34±0.57b	9.63±0.59b	9.04±0.66b	14.99±3.65a	63

变量 Variables	高寒荒漠 Alpine desert	高寒草原 Alpine steppe	高寒草甸 Alpine meadow	高寒湿地 Alpine wetland	变异系数 Coefficient of variation (CV, %)
植物丰富度Plant richness	8.54±0.45ab	7.92±0.21bc	9.54±0.65a	6.78±0.78c	24
香农-威纳指数Shannon-Wiener index	1.93±0.05a	1.89±0.03a	2.06±0.09a	1.65±0.11b	14
土壤含水量SWC （%）	4.39±0.44d	14.06±0.38c	22.60±1.03b	37.30±2.82a	68
土壤pH Soil pH	8.88±0.02a	8.18±0.02b	8.07±0.05b	8.19±0.09b	4
土壤有机碳SOC (g·kg^-1)	5.83±0.85c	27.68±5.10b	58.75±3.55a	53.19±5.51a	73
可溶性有机碳DOC (mg·kg^-1)	68.04±1.63c	119.51±6.46b	160.34±5.16a	156.45±4.17a	33
土壤全氮TN (g·kg^-1)	0.75±0.07c	2.84±0.46b	5.00±0.35a	5.28±0.43a	66
土壤全磷TP (g·kg^-1)	0.25±0.01b	0.51±0.02a	0.51±0.02a	0.49±0.03a	29
土壤有效氮AN (mg·kg^-1)	11.48±0.50c	58.84±2.12b	78.15±5.22a	73.57±11.68a	60
土壤有效磷AP (mg·kg^-1)	2.62±0.46c	6.39±0.44b	8.80±0.42a	5.80±0.90b	49
有效氮磷比N∶P	5.34±0.57b	9.63±0.59b	9.04±0.66b	14.99±3.65a	63

类型Type	F	P
高寒荒漠/高寒草原Alpine desert/alpine steppe	4.89	0.001
高寒荒漠/高寒草甸Alpine desert/alpine meadow	3.87	0.001
高寒荒漠/高寒湿地Alpine desert/alpine wetland	7.03	0.001
高寒草原/高寒草甸Alpine steppe/alpine meadow	3.00	0.002
高寒草原/高寒湿地Alpine steppe/alpine wetland	8.11	0.001
高寒草甸/高寒湿地Alpine meadow/alpine wetland	5.67	0.001

类型Type	F	P
高寒荒漠/高寒草原Alpine desert/alpine steppe	4.89	0.001
高寒荒漠/高寒草甸Alpine desert/alpine meadow	3.87	0.001
高寒荒漠/高寒湿地Alpine desert/alpine wetland	7.03	0.001
高寒草原/高寒草甸Alpine steppe/alpine meadow	3.00	0.002
高寒草原/高寒湿地Alpine steppe/alpine wetland	8.11	0.001
高寒草甸/高寒湿地Alpine meadow/alpine wetland	5.67	0.001

项目 Item	OTU丰富度 OTU richness		香农-威纳指数 Shannon-Wiener index		谱系多样性指数 Faith’s phylogentic diversity index
项目 Item	r	P	r	P	r	P
植物丰富度Plant richness	0.48	0.001	-0.03	0.828	0.49	0.000
香农-威纳指数Shannon-Wiener index	0.47	0.001	-0.15	0.309	0.47	0.001
土壤含水量SWC	-0.59	0.000	-0.07	0.657	-0.60	0.000
pH	0.05	0.715	-0.04	0.788	0.07	0.654
土壤有机碳SOC	-0.17	0.248	-0.12	0.403	-0.17	0.243
土壤全氮TN	-0.23	0.109	-0.13	0.385	-0.24	0.104
土壤全磷TP	-0.06	0.697	0.04	0.777	-0.08	0.594
土壤有效磷AP	0.21	0.160	0.24	0.101	0.19	0.197
可溶性有机碳DOC	-0.23	0.124	-0.04	0.809	-0.23	0.112
土壤有效氮AN	-0.05	0.727	0.02	0.912	-0.06	0.676
有效氮磷比N∶P	-0.33	0.024	-0.40	0.005	-0.32	0.027