Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (3): 47-59.DOI: 10.11686/cyxb2020593
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Li-juan GAO1(), Zheng-she ZHANG1, Yu WEN2, Xi-fang ZONG1, Qi YAN1, Li-yan LU1, Xian-feng YI3, Ji-yu ZHANG1()
Received:
2020-12-29
Revised:
2021-02-09
Online:
2022-03-20
Published:
2022-01-15
Contact:
Ji-yu ZHANG
Li-juan GAO, Zheng-she ZHANG, Yu WEN, Xi-fang ZONG, Qi YAN, Li-yan LU, Xian-feng YI, Ji-yu ZHANG. Genome-wide identification and expression analysis of the bHLH transcription factor family in Cenchrus purpureus[J]. Acta Prataculturae Sinica, 2022, 31(3): 47-59.
基因名称Gene name | 上游引物Forward primer (5'-3') | 下游引物Reverse primer (5'-3') |
---|---|---|
CpbHLH002 | CAAGATCAGCAAGATGGACAGG | GGGTGACTCAAGCTCATTCTG |
CpbHLH013 | ACAAGCAAACCAACACTTCTG | GCTCTCCTTTAACACCTTAACCT |
CpbHLH017 | AGCAAGTTGAGTTCTTGTCC | TCTTTGTGCAGGAGTGTAGAC |
CpbHLH019 | TGCGGAAAGGTTGAGAAGG | TGATCTCGTCAAGCATCACC |
CpbHLH025 | ACAAACAAGACAGATAGGGCAG | GAGAGTGGAATATCAGCAACCAG |
CpbHLH030 | ACAAGCAAACCAACACTTCAG | CTCGTTCAGCACCTTGACC |
CpbHLH055 | GTCAAGGTACTAAGCATGAGCA | TCTTCTTCCATCAGCTTTGTG |
CpbHLH156 | AGCAACAGCGAGAATGGC | CAGCAGCTTCATCCTCTGG |
CpbHLH158 | AGCTGGTTGCTGATATTCCAC | TCTTCCATCAGCTTTGCAACC |
CpEE1a | ACTACGAGCAAGAGTTGGAAAC | TGGATGGCTGGAAGAGGAC |
Table 1 Primer sequences used in real-time quantitative PCR
基因名称Gene name | 上游引物Forward primer (5'-3') | 下游引物Reverse primer (5'-3') |
---|---|---|
CpbHLH002 | CAAGATCAGCAAGATGGACAGG | GGGTGACTCAAGCTCATTCTG |
CpbHLH013 | ACAAGCAAACCAACACTTCTG | GCTCTCCTTTAACACCTTAACCT |
CpbHLH017 | AGCAAGTTGAGTTCTTGTCC | TCTTTGTGCAGGAGTGTAGAC |
CpbHLH019 | TGCGGAAAGGTTGAGAAGG | TGATCTCGTCAAGCATCACC |
CpbHLH025 | ACAAACAAGACAGATAGGGCAG | GAGAGTGGAATATCAGCAACCAG |
CpbHLH030 | ACAAGCAAACCAACACTTCAG | CTCGTTCAGCACCTTGACC |
CpbHLH055 | GTCAAGGTACTAAGCATGAGCA | TCTTCTTCCATCAGCTTTGTG |
CpbHLH156 | AGCAACAGCGAGAATGGC | CAGCAGCTTCATCCTCTGG |
CpbHLH158 | AGCTGGTTGCTGATATTCCAC | TCTTCCATCAGCTTTGCAACC |
CpEE1a | ACTACGAGCAAGAGTTGGAAAC | TGGATGGCTGGAAGAGGAC |
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