Seasonal shift of rumen bacteria quantity of grazing Tibetan sheep and forage nutrition by grazing sheep

Abstract

Abstract: In this study, real time PCR were used to monitor changed quantity of bacteria in rumens of 12 Tibetan sheep with seasonal shift, by collecting rumen content of grazing Tibetan sheep in alpine meadow in spring, summer, autumn and winter, respectively. 1)The quantity of bacteria in grazing Tibetan sheep rumen did not have a significantly change between winter and autumn, and that of spring decreased 2.38, 2.23-fold of autumn and winter, meanwhile, that of summer was significantly higher 5.56-fold, 2.35-fold and 2.49-fold than that of autumn, winter and spring;2)Intake of the grazing sheep in summer was significantly higher 1.98-fold, 1.61-fold and 1.80-fold than that of spring, winter, autumn. Intake of the grazing sheep and quantity of bacteria in rumens of the grazing sheep were decreased with fibre increased of fodder. The quantity of bacteria in rumens of Tibetan sheep changed, which result from fodder, season shift. 3)Real time PCR validated for specific detection and quantification of bacteria in rumens of Tibetan sheep with seasonal shift.

CLC Number:

S826

DAN Rui-fang, ZHANG Hai-tao, LONG Rui-jun, DING Xue-zhi, ZHANG Xin. Seasonal shift of rumen bacteria quantity of grazing Tibetan sheep and forage nutrition by grazing sheep[J]. Acta Prataculturae Sinica, 2009, 18(1): 100-104.

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