Construction of plant expression vectors containing two anti-fungal and one anti-herbicide genes and their expression in transgenic tobacco

Abstract

Abstract: A Chitinase gene (Chi) and a β-1, 3-glucanase gene (Glu) were amplified from pET/Chi and pET/Glu plasmids respectively, and connected by eight neutral amino acids, to form a fusion gene (Chi-linker-Glu) which was inserted into pAHC25 by replacing the GUS gene. The expression cassettes Ubi-Chi-linker-Glu-nos and Ubi-bar-nos from recombinant plasmid pAHC25-C-G were inserted into vector pBI121 to obtain plant expression vector pBIb-CG. Leaf discs of tobacco were infected by Agrobacterium tumefaciens strain EHA105 containing this plant expression vector. The fusion gene Chi-linker-Glu was shown to have been integrated into the tobacco genome by PCR and Southern blotting analysis. In vitro testing of transgenic plants showed that they had resistance to Tricho derma viride and Fusarium solanif sppisii. The fusion gene Chi-linker-Glu was therefore expressed efficiently in transgenic tobacco to give disease-resistance.

CLC Number:

S572.032

JIA Xiao-xia, ZHANG Jin-wen, WANG Han-ning, MA Huai-yi, LIANG Hui-guang. Construction of plant expression vectors containing two anti-fungal and one anti-herbicide genes and their expression in transgenic tobacco[J]. Acta Prataculturae Sinica, 2009, 18(1): 86-93.

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