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Acta Prataculturae Sinica ›› 2009, Vol. 18 ›› Issue (6): 59-64.

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Research on tissue culture and medium of Nitraria tangutorum

GUO Ye-hong, LIN Hai-ming, WU Rui   

  1. Agriculture college, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2009-05-02 Online:2009-12-20 Published:2009-12-20

Abstract: Young stem segments and leaves of Nitraria tangutorum were selected to investigate suitable media and plant regeneration techniques for explants. Immature stems were a good source for explants of tufted shoots, and cultivation at 20℃ can reduce contamination rates of tufted shoots. Leaves were good explants of callus induction as well and a lower concentration of 2,4-D was better for callus than a higher concentration. The optimum multiplication medium for N. tangutorum was MS+6-BA 2 mg/L+NAA 1.00 mg/L and tufted shoots formed from axil buds were the main multiplication method. The optimum medium for root formation was 1/2 MS+KT 1 mg/L+IBA 0.5 mg/L and the optimum medium for callus induction was MS+2.4-D 0.5-1.0 mg/L.

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